From Analytical Profiling to Liposomal Delivery: Cannabinol as a Model for Antioxidant Encapsulation and Diffusion...

Area of Science:

• Pharmacology
• Materials Science
• Dermatology

Background:

• Cannabinoids possess potential therapeutic properties, including antioxidant effects.
• Efficient delivery systems are crucial for topical cannabinoid applications.
• Cannabinol (CBN) is a promising cannabinoid with unexplored antioxidant and delivery potential.

Purpose of the Study:

• To evaluate the antioxidant capacity of five distinct cannabinoids, focusing on CBN.
• To formulate CBN into liposomes for improved delivery.
• To assess the physicochemical properties, stability, and diffusion of CBN-loaded liposomes for dermal applications.

Main Methods:

• Structural characterization using mass spectrometry (MS) and nuclear magnetic resonance (NMR).
• In vitro antioxidant activity assays (DPPH, hydroxyl, superoxide radicals).
• Liposome formulation using soy lecithin and characterization of colloidal properties (size, membrane order).
• Electron paramagnetic resonance (EPR) imaging for diffusion studies in a semi-solid model.

Main Results:

• CBN demonstrated the most potent and balanced radical scavenging activity among tested cannabinoids.
• CBN-loaded liposomes exhibited favorable colloidal properties (approx. 122.9 nm) and enhanced bilayer stability.
• Liposomal CBN retained significant antioxidant capacity and showed superior diffusion compared to free CBN in a model system.

Conclusions:

• CBN possesses significant antioxidant potential.
• Soy lecithin liposomes are a suitable delivery system for CBN, enhancing its stability and diffusion.
• CBN-loaded liposomes show promise for dermal antioxidant applications, warranting further investigation in skin models.