Balancing the Cellular Inflammatory-Homeostatic Axis Through Natural Ingredient Supplementation

Area of Science:

• Immunology
• Cell Biology
• Nutritional Science

Background:

• Inflammation significantly disrupts human homeostasis.
• Macrophages play a critical role in the inflammatory response.
• Dietary supplements can provide nutrients to support healthy lifestyles.

Purpose of the Study:

• Investigate the effects of a multi-ingredient dietary supplement on human macrophages.
• Assess the supplement's impact on inflammatory pathways and cellular functions.
• Determine the presence and bioavailability of supplement ingredients within cells.

Main Methods:

• THP-1 cells differentiated into M1/M2 macrophages.
• Assays for cell migration, gene expression (qRT-PCR), cytokine release (ELISA), and pyroptosis (LDH).
• HPLC-PDA-MS/MS and GC-MS used for ingredient analysis.

Main Results:

• Supplement promoted M2 macrophage polarization and migration while reducing M1 migration.
• Reduced NF-κB activation, COX-2 expression, and cytokine release in an LPS-induced inflammation model.
• Decreased granulocyte adhesion and oxidative stress; confirmed ingredient presence in cells.

Conclusions:

• The multi-ingredient supplement modulates macrophage phenotypes and inflammatory responses.
• The formulation's natural active ingredients contribute to maintaining cellular homeostasis.
• Demonstrated potential for dietary supplements in managing inflammation and supporting health.