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Multi-Omics and Molecular Docking Studies on Caffeine for Its Skin Rejuvenating Potentials
Peng Shu1, Nan Zhao1, Qi Zhou1
1HBN Research Institute and Biological Laboratory, Shenzhen Hujia Technology Co., Ltd., Shenzhen 518000, China.
Pharmaceuticals (Basel, Switzerland)
|August 28, 2025
Summary
Caffeine (CA) effectively repairs UV-induced skin aging by modulating ferroptosis and metabolic pathways. This study reveals CA
Area of Science:
- Dermatology
- Biochemistry
- Molecular Biology
Background:
- Ultraviolet (UV) radiation induces skin aging through complex molecular mechanisms.
- Caffeine (CA) shows potential in mitigating UV-induced skin damage, but its precise mechanisms remain unclear.
- Understanding CA's role in regulating gene expression, metabolites, and signaling pathways is crucial.
Purpose of the Study:
- To investigate the reparative effects of caffeine against UV-induced skin aging.
- To explore the involvement of the ferroptosis pathway in caffeine's anti-aging effects.
- To elucidate the molecular mechanisms underlying caffeine's protective action on skin.
Main Methods:
- In vitro cell experiments using HaCaT cells.
- In vivo studies utilizing a UV-aged mouse skin model.
- Multi-omics analysis, network pharmacology, and molecular docking techniques.
Main Results:
- Caffeine enhanced cell vitality, proliferation, and mitochondrial function while reducing reactive oxygen species.
- CA modulated peroxisome proliferator-activated receptor pathway and repaired UVB-induced cytoskeletal damage.
- Multi-omics data indicated caffeine mitigates aging by regulating metabolic and ferroptosis pathways, reducing lipid peroxidation and key ferroptosis proteins.
Conclusions:
- Caffeine demonstrates significant effectiveness in reducing UV-induced skin aging.
- The study elucidates caffeine's potential anti-aging mechanism through the modulation of ferroptosis pathways.
- Molecular docking confirms strong interactions between caffeine and relevant proteins, supporting its therapeutic potential.
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