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Related Experiment Video

Updated: Sep 9, 2025

Infection of Primary Nasal Epithelial Cells Grown at an Air-Liquid Interface to Characterize Human Coronavirus-Host Interactions

Infection of Primary Nasal Epithelial Cells Grown at an Air-Liquid Interface to Characterize Human Coronavirus-Host Interactions

Published on: September 22, 2023

4-Hydroxychalcone Inhibits Human Coronavirus HCoV-OC43 by Targeting EGFR/AKT/ERK1/2 Signaling Pathway.

Yuanyuan Huang^1,2, Jieyu Li^1,2, Qiting Luo²

¹School of Pharmacy, Guangdong Pharmaceutical University, Guangzhou 510006, China.

|August 28, 2025

Summary

4-hydroxychalcone (4HCH) shows strong antiviral effects against human coronavirus HCoV-OC43. This compound inhibits viral replication by targeting the epidermal growth factor receptor (EGFR) pathway, offering potential as a new coronavirus treatment.

Keywords:

4-hydroxychalcone EGFR EGFR/AKT/ERK1/2 signaling pathway antiviral activity flavonoids human coronavirus

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Titration of Human Coronaviruses Using an Immunoperoxidase Assay

Titration of Human Coronaviruses Using an Immunoperoxidase Assay

Published on: April 28, 2008

Area of Science:

Virology
Pharmacology
Immunology

Background:

Human coronaviruses pose a significant threat to global health.
Effective antiviral therapies for coronaviruses are urgently needed.

Purpose of the Study:

To investigate the antiviral activity of 4-hydroxychalcone (4HCH) against human coronavirus HCoV-OC43.
To elucidate the mechanism of action of 4HCH against HCoV-OC43 infection.

Main Methods:

In vitro assays to assess cytopathic effect inhibition, viral protein, and RNA levels.
In vivo studies using HCoV-OC43-infected suckling mice to evaluate survival rates.
Analysis of the epidermal growth factor receptor (EGFR) signaling pathway and pro-inflammatory cytokine production.

Main Results:

4HCH significantly inhibited HCoV-OC43 cytopathic effects, viral load, and increased survival in infected mice.
4HCH targets early viral infection stages by binding to EGFR, inhibiting the EGFR/AKT/ERK1/2 pathway, and suppressing viral replication.
4HCH reduced pro-inflammatory cytokine and chemokine production in infected cells and animal models.

Conclusions:

4HCH demonstrates potent in vitro and in vivo antiviral activity against HCoV-OC43.
EGFR is identified as a promising host target for developing novel antiviral drugs.
4HCH is a potential therapeutic candidate for treating human coronavirus infections.