Related Experiment Video
Updated: May 6, 2026
13:36
Transnuclear Mice with Pre-defined T Cell Receptor Specificities Against Toxoplasma gondii Obtained Via SCNT
Published on: September 30, 2010
14.3K
Immune Responses and Replication of Rescued Torque Teno Virus (TTSuV1) in Mice
Md-Tariqul Islam1,2, Brett Webb3, Sheela Ramamoorthy1
1Department of Microbiological Sciences, North Dakota State University, Fargo, ND 58102, USA.
Viruses
|August 28, 2025
Summary
Swine Torque Teno Viruses (TTSuV) can cause disease. This study developed a mouse model showing TTSuV1 infects mice, triggers immune responses, and impacts lymphocytes, aiding TTV immunopathogenesis research.
Area of Science:
- Virology
- Immunology
- Animal Models
Background:
- Torque Teno Viruses (TTVs) are widespread in mammals.
- Swine TTVs (TTSuVs) are emerging as potential swine pathogens.
- Lack of robust models hindered TTV-host interaction studies.
Purpose of the Study:
- To establish an in vivo model for studying TTSuV infection and immunopathogenesis.
- To investigate the infectivity and immune response to recombinant TTSuV1 in mice.
Main Methods:
- Infection of C57BL/J6 mice with recombinant TTSuV1.
- Detection of viral DNA and antigen in tissues and lymphocytes.
- Assessment of immune responses, including antibody production and mitogenic stimulation.
- In vitro culture of TTSuV1 in PK-15 cells.
Main Results:
- Infected mice developed neutralizing antibodies and had detectable viral DNA in blood and lungs.
- TTSuV1 productively infected PK-15 cells in vitro.
- Viral antigen was found in T and B lymphocytes.
- Mice showed splenic hyperplasia, lymphopenia, and diminished immune responses.
Conclusions:
- Recombinant TTSuV1 is infective in mice, inducing measurable immune responses.
- This study provides the first experimental evidence of TTSuV1 infectivity and immunomodulation in a laboratory animal model.
- The developed mouse model is crucial for future TTV immunopathogenesis research.
