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Related Concept Videos

Immunoprecipitation01:20

Immunoprecipitation

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Immunoprecipitation, or IP, is a widely used technique that employs protein-antibody interactions to isolate proteins or protein complexes in their native state for studying protein-protein interactions, quaternary structures, or supramolecular complexes. Various modifications of the technique, including chromatin IP, cross-linking IP, and fluorescence IP, are commonly used.
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Chromatin immunoprecipitation, or ChIP, is an antibody-based technique used to identify sites on DNA that bind to transcription factors of interest or histone proteins. It also helps determine the type of histone modifications such as acetylation, phosphorylation, or methylation.
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The first human genome sequencing project cost $2.7 billion and was declared complete in 2003, after 15 years of international cooperation and collaboration between several research teams and funding agencies. Today, with the advent of next-generation sequencing technologies, the cost and time of sequencing a human genome have dropped over 100 fold.
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Related Experiment Video

Updated: Sep 9, 2025

Interactome-Seq: A Protocol for Domainome Library Construction, Validation and Selection by Phage Display and Next Generation Sequencing
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[Advances in phage immunoprecipitation sequencing technology].

Yuhao Zhu1,2,3,4, Wenlong Zhu1,2,3,4, Yujie Lai1,2,3,4

  • 1College of Veterinary Medicine, Huazhong Agricultural University, Wuhan 430070, Hubei, China.

Sheng Wu Gong Cheng Xue Bao = Chinese Journal of Biotechnology
|August 28, 2025
PubMed
Summary

Phage immunoprecipitation sequencing (PhIP-Seq) is a cost-effective method to analyze protein-peptide binding. This high-throughput technology screens peptide targets for hundreds of proteins, aiding in disease detection and biomarker discovery.

Keywords:
diagnostic techniquespeptide libraryphage immunoprecipitation sequencing (PhIP-Seq)vaccine development

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Area of Science:

  • Biotechnology
  • Molecular Biology
  • Immunology

Background:

  • Phage immunoprecipitation sequencing (PhIP-Seq) is a powerful technique for analyzing protein-peptide interactions.
  • It utilizes oligonucleotide library synthesis (OLS) to create proteome-scale peptide libraries displayed on phages.

Purpose of the Study:

  • To systematically describe the development and applications of PhIP-Seq.
  • To provide a comprehensive understanding of PhIP-Seq's potential in various scientific fields.

Main Methods:

  • PhIP-Seq involves synthesizing peptide libraries on phages using OLS.
  • Target proteins (e.g., antibodies) are used to immunoprecipitate specific library phages.
  • High-throughput DNA sequencing analyzes the precipitated phages.

Main Results:

  • PhIP-Seq enables high-throughput screening of peptide targets against hundreds of proteins or pathogens.
  • The method has demonstrated success in disease detection, autoimmune biomarker screening, vaccine development, and allergen detection.
  • PhIP-Seq is recognized as a high-throughput diagnostic technology.

Conclusions:

  • PhIP-Seq is a versatile and low-cost method for analyzing protein-peptide binding.
  • Its applications span diagnostics, biomarker discovery, and therapeutic development.
  • Further exploration of PhIP-Seq's capabilities promises advancements in various scientific domains.