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Related Experiment Video

Updated: Sep 9, 2025

Human In Vitro Suppression as Screening Tool for the Recognition of an Early State of Immune Imbalance

Human In Vitro Suppression as Screening Tool for the Recognition of an Early State of Immune Imbalance

Published on: July 22, 2011

Assessing Human Treg Suppression at Single-Cell Resolution Using Mass Cytometry.

Jonas Nørskov Søndergaard¹, Janyerkye Tulyeu¹, David Priest¹

¹Human Immunology Team, Center for Infectious Disease Education and Research (CiDER), The University of Osaka, Suita, Japan.

|August 28, 2025

Summary

This summary is machine-generated.

Regulatory T cells (Tregs) maintain immune balance. This study presents a high-dimensional assay to profile Treg suppression in complex blood cell systems, enhancing immune research and drug evaluation.

Keywords:

CyTOF Immune profiling Mass cytometry PBMC Regulatory T cells Single-cell analysis Suppression assay

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Last Updated: Sep 9, 2025

Human In Vitro Suppression as Screening Tool for the Recognition of an Early State of Immune Imbalance

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Published on: July 22, 2011

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Published on: January 3, 2025

Area of Science:

Immunology
Cell Biology
Mass Cytometry

Background:

Regulatory T cells (Tregs) are crucial for immune homeostasis.
Current Treg suppression assays lack physiological relevance and multiplexing capabilities.

Purpose of the Study:

To develop a high-dimensional, single-cell assay for profiling Treg-mediated suppression within autologous peripheral blood mononuclear cells (PBMCs).
To enable integrated analysis of Treg function and immune responses in a complex cellular environment.

Main Methods:

Isolation of Tregs via cell sorting.
Reintroduction of Tregs into autologous PBMCs at defined ratios.
Utilizing a 52-marker mass cytometry (CyTOF) panel to quantify cell division and phenotypic responses.

Main Results:

Quantification of Treg-mediated suppression at single-cell resolution within PBMCs.
High-dimensional phenotyping and proliferation tracking across multiple immune subsets.
Demonstration of assay compatibility with patient-derived samples and drug perturbation.

Conclusions:

The developed CyTOF assay provides a physiologically relevant and high-dimensional method for assessing Treg function.
This approach supports translational immunology research, including patient profiling and drug evaluation.