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One mechanism of spleen-kidney yang deficiency IBS-D: intestinal microbiota affect ATPase

Qi Long1,2, Liwen Li1,2, Na Deng1,2

  • 1School of Traditional Chinese Medicine, Hunan University of Chinese Medicine, Changsha, China.

Frontiers in Microbiology
|August 28, 2025
PubMed
Abstract

Insights

This study reveals key changes in gut enzymes and microbial activity in a mouse model of irritable bowel syndrome with diarrhea (IBS-D). These metabolic shifts are linked to spleen-kidney yang deficiency, offering insights into IBS-D mechanisms.

Area of Science:

  • Gastroenterology
  • Microbiology
  • Metabolism

Background:

  • Irritable Bowel Syndrome with Diarrhea (IBS-D) is a common gastrointestinal disorder.
  • Spleen-kidney yang deficiency is a Traditional Chinese Medicine pattern associated with IBS-D.
  • Energy metabolism plays a role in the pathophysiology of IBS-D.

Purpose of the Study:

  • To investigate the impact of adenine and Folium senna combined with restraint tail-clamping stress on the intestinal microbiota of mice with spleen-kidney yang deficiency type IBS-D.
  • To explore the relationship between energy metabolism and intestinal microbiota in IBS-D.

Main Methods:

  • Establishment of a spleen-kidney yang deficiency IBS-D mouse model using adenine, Folium senna, and restraint tail-clamping stress.
  • Enzyme-linked immunosorbent assay (ELISA) to measure Na+-K+-ATPase and Ca2+-Mg2+-ATPase activity.
  • Spectrophotometry to assess intestinal digestive enzyme activity and microbial activity (FDA hydrolysis).
  • 16S rRNA sequencing to identify intestinal microbiota composition.

Main Results:

  • Model mice showed significantly decreased Na+-K+-ATPase and Ca2+-Mg2+-ATPase activity.
  • Intestinal enzyme activity alterations included increased lactase and amylase, and decreased sucrase.
  • Intestinal microbial activity was significantly enhanced in model mice.
  • Specific bacterial genera (Heminiphilus, Clostridium, Desulfovibrio, Pediococcus) correlated with enzyme activities.

Conclusions:

  • Reduced ATPase activities, altered digestive enzyme profiles, and enhanced microbial activity are potential mechanisms in spleen-kidney yang deficiency IBS-D.
  • These metabolic and microbial changes contribute to the development of IBS-D.
  • Further research into energy metabolism and gut microbiota interactions in IBS-D is warranted.
Keywords:
ATPaseenergy metabolismintestinal digestive enzymesintestinal microbiotaspleen-kidney yang deficiency IBS-D

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