Related Experiment Video
Updated: Sep 9, 2025
13:24
An Optimized Enrichment Technique for the Isolation of Arthrobacter Bacteriophage Species from Soil Sample Isolates
Published on: April 9, 2015
19.8K
Complete genome sequence of Arthrobacter phage Tiff81.
Huimin Xian1, Alyashanti L Green1, Christopher J Curran1
1Tufts University, Medford, Massachusetts, USA.
Microbiology Resource Announcements
|August 28, 2025
Summary
A novel bacteriophage, Tiff81, was identified from soil, infecting Arthrobacter globiformis. This siphoviral phage possesses a large genome and belongs to the Actinobacteriophage cluster AY.
Area of Science:
- Microbiology
- Virology
- Genomics
Background:
- Bacteriophages are viruses that infect bacteria and play crucial roles in microbial ecosystems.
- Actinobacteriophages are a diverse group of viruses that infect Actinobacteria, an important phylum of bacteria.
Purpose of the Study:
- To isolate and characterize a novel bacteriophage infecting Arthrobacter globiformis.
- To determine the genomic features and taxonomic placement of the isolated bacteriophage.
Main Methods:
- Isolation of bacteriophage Tiff81 from soil samples.
- Morphological characterization using electron microscopy (siphoviral morphology).
- Genomic sequencing and analysis (50,530 bp genome, 90 predicted genes, 62.6% GC content).
Main Results:
- Tiff81 was successfully isolated and identified as a siphoviral bacteriophage.
- The genome size and GC content were determined.
- Tiff81 was classified as a member of Actinobacteriophage cluster AY, alongside 37 other phages.
Conclusions:
- Tiff81 represents a new addition to the known diversity of Actinobacteriophages.
- Its characterization contributes to understanding phage-host interactions within Actinobacteria.
- The genomic data provides a foundation for further functional studies.
Related Concept Videos
01:26DNA Bacteriophages
140
Bacteriophages, or phages, are viruses that specifically infect bacteria, utilizing their genetic material to hijack host cellular machinery for replication. DNA bacteriophages employ single-stranded DNA (ssDNA) or double-stranded DNA (dsDNA) genomes. These phages exhibit diverse replication strategies and host interactions, influencing their ecological roles and applications in biotechnology and medicine.ssDNA BacteriophagesssDNA phages, with their small genomes, utilize unique strategies to...
140
01:20Viral Replication: Lytic Cycle
217
Bacteriophages, or phages, are viruses that specifically infect bacteria. Among them, T-even bacteriophages, such as T4, exhibit a well-characterized lytic replication cycle in Escherichia coli (E. coli). This process ensures the rapid proliferation of the virus while ultimately leading to the destruction of the bacterial host.Attachment and DNA InjectionThe infection process begins with the recognition and binding of the T4 phage to the E. coli cell surface. Tail fibers of the phage...
217
01:29Viruses of Archaea
75
Archaeal viruses play a crucial role in the ecosystems of extremophilic archaea, particularly those belonging to the phyla Euryarchaeota and Crenarchaeota. By shaping host evolution and facilitating gene transfer, these viruses influence microbial communities and contribute to genetic diversity in extreme environments. The archaea they infect thrive in acidic hot springs and hydrothermal vents characterized by high temperatures and low pH. Archaeal viruses exhibit remarkable structural...
75
01:30Lytic Cycle of Bacteriophages
71.9K
Bacteriophages, also known as phages, are specialized viruses that infect bacteria. A key characteristic of phages is their distinctive “head-tail” morphology. A phage begins the infection process (i.e., lytic cycle) by attaching to the outside of a bacterial cell. Attachment is accomplished via proteins in the phage tail that bind to specific receptor proteins on the outer surface of the bacterium. The tail injects the phage’s DNA genome into the bacterial cytoplasm. In the...
71.9K
00:46Genomic DNA in Prokaryotes
44.5K
The genome of most prokaryotic organisms consists of double-stranded DNA organized into one circular chromosome in a region of cytoplasm called the nucleoid. The chromosome is tightly wound, or supercoiled, for efficient storage. Prokaryotes also contain other circular pieces of DNA called plasmids. These plasmids are smaller than the chromosome and often carry genes that confer adaptive functions, such as antibiotic resistance.
Genomic Diversity in Bacteria
Although bacterial genomes are much...
Genomic Diversity in Bacteria
Although bacterial genomes are much...
44.5K
01:24Bacterial Phylum Tenericutes
88
The phylum Tenericutes, which includes the single class Mollicutes, comprises bacteria that lack cell walls. The term "Mollicutes" derives from the Latin word mollis, meaning "soft." These organisms are among the smallest known and are commonly referred to as mycoplasmas due to the prominence of the genus Mycoplasma, which includes well-known human pathogens. Despite their inability to stain gram-positively (a result of their lack of cell walls), mycoplasmas are phylogenetically related to the...
88