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Updated: Sep 9, 2025

Isolation and Culture of Primary Mouse Keratinocytes from Neonatal and Adult Mouse Skin
Published on: July 14, 2017
Skin Lipid-Microbe Interplay Links Staphylococcus hominis to Barrier Control in Adult Atopic Dermatitis
Madhumita Bhattacharyya1,2, Felix Lauffer3,4, Manja Jargosch4,5
1Environmental Medicine - Institute of Environmental Medicine and Integrative Health, Faculty of Medicine, University of Augsburg and University Hospital of Augsburg, Augsburg, Germany.
Background:
Skin surface lipids and commensal microbes are essential for the epidermal barrier, but their mutual interactions remain poorly understood.
Methods:
We conducted high-resolution shotgun lipidomics of tape strips from lesional and non-lesional atopic dermatitis (AD) skin and healthy controls. Lipidomic data were integrated with 16S amplicon sequencing to construct lipid-microbe interaction networks.
Results:
AD skin showed disease-specific lipid-microbe correlations, with less diverse interactions in lesional compared to non-lesional and healthy skin. Staphylococcus hominis (S. hominis) negatively correlated with non-hydroxy-dehydrosphingosine (NdS) 18:0;2/24:0;0 and positively with diacylglycerol (DAG) 18:1;0_18:1;0 and DAG 16:0;0_18:1;0. In vitro co-cultures of reconstructed human epidermis (RHE) with AD skin-derived T cell supernatant (TCS) and S. hominis reduced RHE thickness, spongiosis, and NdS 18:0;2/24:0;0 levels. Furthermore, S. hominis directly lowered NdS 18:0;2/24:0;0 levels in lesional AD skin tape samples, and reversed type 2 inflammation and lipid metabolism gene expression in TCS-stimulated RHE.
Conclusions:
These findings identify S. hominis as a key regulator of lipid-microbe interactions in AD, influencing epidermal inflammation and differentiation.
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