Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Bio-Oss<sup>®</sup>/Avitene™ composite scaffold promotes maxillofacial bone regeneration via early osteoimmunomodulation of BM-MSCs: an <i>in vitro</i> and clinical study.

Frontiers in bioengineering and biotechnology·2026
Same author

Modulation of immune responses by elexacaftor/tezacaftor/ivacaftor therapy in cystic fibrosis: data from a compassionate use program.

Respiratory research·2026
Same author

Peptide Nucleic Acid Probes for MicroRNA Detection: Mg<sup>2+</sup> Ion Effect, Surface Hybridization, and Surface Plasmon Resonance Biosensing.

ACS measurement science au·2025
Same author

Black Garlic: Evolution of the Chemical Composition and Broad Biological Activities.

Journal of agricultural and food chemistry·2025
Same author

Beyond boundaries: how autonomy and technology promote work engagement and well-being in remote workers.

Frontiers in public health·2025
Same author

Impact of Chromosomal Structural Rearrangements on IVF Laboratory Outcomes in PGT-SR Cycles: A Propensity Score Matching-Based Study.

Life (Basel, Switzerland)·2025

Related Experiment Video

Updated: Sep 9, 2025

Chromosome Screening of Human Preimplantation Embryos by Using Spent Culture Medium: Sample Collection and Chromosomal Ploidy Analysis
12:32

Chromosome Screening of Human Preimplantation Embryos by Using Spent Culture Medium: Sample Collection and Chromosomal Ploidy Analysis

Published on: September 7, 2021

2.2K

Non-Invasive Preimplantation Genetic Testing: Cell-Free DNA Detection in Embryo Culture Media Using a Plasmonic

Noemi Bellassai1,2, Anil Biricik3, Matteo Surdo3

  • 1Department of Chemical Sciences, University of Catania, Viale Andrea Doria 6, 95125 Catania, Italy.

Analytical Chemistry
|August 28, 2025
PubMed
Summary
This summary is machine-generated.

This study introduces a novel noninvasive preimplantation genetic testing (niPGT) method for embryo genetic screening. The advanced biosensor detects single-point mutations in cell-free DNA, improving embryo selection for in vitro fertilization (IVF).

More Related Videos

FISH for Pre-implantation Genetic Diagnosis
07:34

FISH for Pre-implantation Genetic Diagnosis

Published on: February 23, 2011

37.0K
A Novel Culture Model for Human Pluripotent Stem Cell Propagation on Gelatin in Placenta-conditioned Media
07:33

A Novel Culture Model for Human Pluripotent Stem Cell Propagation on Gelatin in Placenta-conditioned Media

Published on: August 3, 2015

8.0K

Related Experiment Videos

Last Updated: Sep 9, 2025

Chromosome Screening of Human Preimplantation Embryos by Using Spent Culture Medium: Sample Collection and Chromosomal Ploidy Analysis
12:32

Chromosome Screening of Human Preimplantation Embryos by Using Spent Culture Medium: Sample Collection and Chromosomal Ploidy Analysis

Published on: September 7, 2021

2.2K
FISH for Pre-implantation Genetic Diagnosis
07:34

FISH for Pre-implantation Genetic Diagnosis

Published on: February 23, 2011

37.0K
A Novel Culture Model for Human Pluripotent Stem Cell Propagation on Gelatin in Placenta-conditioned Media
07:33

A Novel Culture Model for Human Pluripotent Stem Cell Propagation on Gelatin in Placenta-conditioned Media

Published on: August 3, 2015

8.0K

Area of Science:

  • Reproductive Biology
  • Biotechnology
  • Genetics

Background:

  • In vitro fertilization (IVF) requires accurate embryo genetic assessment for successful implantation.
  • Current methods for preimplantation genetic testing often involve invasive embryo biopsy.
  • Noninvasive biomarkers in spent culture medium offer potential for genetic evaluation.

Purpose of the Study:

  • To develop a noninvasive preimplantation genetic testing (niPGT) method.
  • To detect single-point mutations in cell-free DNA (cfDNA) from embryo culture medium.
  • To enhance embryo selection for IVF by assessing genetic health without biopsy.

Main Methods:

  • Utilized a superparamagnetic particles-enhanced surface plasmon resonance (SPR) imaging biosensor.
  • Employed biotinylated locked nucleic acid (LNA) probes for specific cfDNA capture.
  • Developed an assay to detect β-globin gene mutations related to β-thalassemia using peptide-nucleic acid (PNA) probes.

Main Results:

  • Achieved attomolar-level detection of heterozygous or homozygous mutated cfDNA.
  • Demonstrated detection in minimal sample volumes (10 μL) with no dilution.
  • Obtained reliable results within 2 hours, showing high sensitivity and specificity.

Conclusions:

  • The developed niPGT approach enables rapid, biopsy-free genetic assessment of embryos.
  • This plasmonic-based biosensor significantly improves confidence in identifying unaffected embryos.
  • Facilitates informed embryo selection for implantation in IVF procedures.