Related Experiment Video
Updated: May 8, 2026

Hybrid µCT-FMT imaging and image analysis
Published on: June 4, 2015
Development of a method for evaluation of absorbed doses in preclinical in vivo μCT
Richard T Deyhle1, Christian Bernhardsson1, Lars E Olsson1,2
1Medical Radiation Physics, Department of Translational Medicine, Lund University, Skåne University Hospital Malmö, Inga-Marie Nilssons gata 49, 205 02 Malmö, Sweden.
Abstract:
In vivo preclinical X-ray micro-computed tomography (μCT) imaging is widely used to obtain three-dimensional anatomical information of small animals. However, the potential for radiation exposure to influence experimental outcomes necessitates accurate dose estimation. The aim of this study was to develop a robust and reproducible method for estimating and evaluating absorbed doses in small animals undergoing preclinical in vivo μCT imaging. Absorbed doses were measured at four separate positions within a dedicated polymethyl methacrylate phantom using MCP-N (LiF, Cu, P) thermoluminescence dosemeters (TLDs) and a novel type of optically stimulated luminescence dosemeter (OSLD) made from household salt (NaCl). The findings were benchmarked against the vendor's absorbed dose estimates, revealing a discrepancy between the measured and provided values. The results for the OSLDs followed a similar trend to the TLDs, though significant statistical differences were found between the luminescent dosemeters and the vendor values. In this study, a method for measuring the absorbed doses from μCT systems was presented. The results indicate a need for thorough dose measurements prior to performing longitudinal imaging studies.
Related Concept Videos
Methods for Studying Drug Absorption: In vitro
The diffusion cell method uses a two-compartment cell, including a donor compartment with the drug solution, which simulates the environment where the drug is applied, and a receptor compartment with a buffer solution, which simulates the environment...
Methods for Studying Drug Absorption: In situ
The Doluisio method involves perfusing a prepared segment of a rat's small intestine with a solution of radiolabeled drug and a non-absorbable marker. This helps to differentiate between absorbed and non-absorbed drug concentrations. The intestinal segment is connected at both ends using tubing and syringes,...
Measurement of Bioavailability: Pharmacokinetic Methods
Measurement of Bioavailability: Pharmacodynamic Methods
Determination of Multiple Dosing Parameters: Loading and Maintenance Doses
Therapeutic Drug Monitoring: Drug Analysis Methods

