Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Experimental RNAi02:15

Experimental RNAi

6.2K
RNA interference (RNAi) is a cellular mechanism that inhibits gene expression by suppressing its transcription or activating the RNA degradation process. The mechanism was discovered by Andrew Fire and Craig Mello in 1998 in plants. Today, it is observed in almost all eukaryotes, including protozoa, flies, nematodes, insects, parasites, and mammals. This precise cellular mechanism of gene silencing has been developed into a technique that provides an efficient way to identify and determine the...
6.2K
RNA Interference01:23

RNA Interference

26.4K
RNA interference (RNAi) is a process in which a small non-coding RNA molecule blocks the post-transcriptional expression of a gene by binding to its messenger RNA (mRNA) and preventing the protein from being translated.
This process occurs naturally in cells, often through the activity of genomically-encoded microRNAs. Researchers can take advantage of this mechanism by introducing synthetic RNAs to deactivate specific genes for research or therapeutic purposes. For example, RNAi could be used...
26.4K
RNA Stability01:53

RNA Stability

33.9K
Intact DNA strands can be found in fossils, while scientists sometimes struggle to keep RNA intact under laboratory conditions. The structural variations between RNA and DNA underlie the differences in their stability and longevity. Because DNA is double-stranded, it is inherently more stable. The single-stranded structure of RNA is less stable but also more flexible and can form weak internal bonds. Additionally, most RNAs in the cell are relatively short, while DNA can be up to 250 million...
33.9K
RNA Editing02:23

RNA Editing

9.2K
RNA editing is a post-transcriptional modification where a precursor mRNA (pre-mRNA) nucleotide sequence is changed by base insertion, deletion, or modification. The extent of RNA editing varies from a few hundred bases, in mitochondrial DNA of trypanosomes, to a just single base, in nuclear genes of mammals. Even a single base change in the pre-mRNA can convert a codon for one amino acid into the codon for another amino acid or a stop codon. This type of re-coding can significantly affect the...
9.2K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Ice-assisted cryogenic embrittlement grinding for surfactant-free fabrication of soft nanomaterials.

Materials horizons·2026
Same author

Site-Specifically Modified Circular Ribonucleic Acid Serves as Multitarget miRNA Sponge with Low Immunogenicity.

Journal of the American Chemical Society·2026
Same author

DNA Supramolecular Hydrogel Alters Exosomal microRNA Atlas and Paracrine Secretory Profile of MSCs to Promote Bone Remodeling and Ameliorate Osteoradionecrosis of the Jaw.

Advanced materials (Deerfield Beach, Fla.)·2026
Same author

Decoding human tRNA modifications and crosstalk by enhanced single-read analysis.

Genome biology·2026
Same author

Preparation strategies and biomedical applications of DNA hydrogels.

Chemical science·2026
Same author

Site-Specific and Regioselective Modification of Human Epidermal Growth Factor Receptor 2 via Site-Sculpted DNA Nanorefiners.

Angewandte Chemie (International ed. in English)·2025
Same journal

On-Cell Detection of Polysaccharide One-Bond <sup>1</sup>J<sub>CH</sub> Couplings by Proton-Detected Solid-State NMR.

Journal of the American Chemical Society·2026
Same journal

Correction to "Unraveling the Effects of Fe Incorporation on High-Performance Water-Splitting Photoanodes".

Journal of the American Chemical Society·2026
Same journal

Proximity-Driven Protein Ligation Beyond the Concentration Limit.

Journal of the American Chemical Society·2026
Same journal

GraPhAI: Neural Networks for Solving Centrosymmetric Crystal Structures.

Journal of the American Chemical Society·2026
Same journal

Probing Stage Transition Kinetics in Li-Graphite Intercalation Compounds by Time-Resolved In Situ Solid-State NMR via <sup>13</sup>C Labeling.

Journal of the American Chemical Society·2026
Same journal

Dynamic Covalent Programming at DNA Base-Pairing Interfaces.

Journal of the American Chemical Society·2026
See all related articles

Related Experiment Video

Updated: Sep 9, 2025

Large-scale Production of Recombinant RNAs on a Circular Scaffold Using a Viroid-derived System in Escherichia coli
10:38

Large-scale Production of Recombinant RNAs on a Circular Scaffold Using a Viroid-derived System in Escherichia coli

Published on: November 30, 2018

9.8K

A Computationally Optimized Ribonucleic Acid Circularization Strategy without Byproducts.

Ruofan Chen1, Yuan Zhuang1,2, Li Zhang3

  • 1Engineering Research Center of Advanced Rare Earth Materials (Ministry of Education), Department of Chemistry, Tsinghua University, Beijing 100084, China.

Journal of the American Chemical Society
|August 28, 2025
PubMed
Summary
This summary is machine-generated.

Researchers developed a purification-free method for creating circular messenger RNA (mRNA) with enhanced stability and protein translation. This advance simplifies production and boosts potential for mRNA therapeutics.

More Related Videos

Use of Alu Element Containing Minigenes to Analyze Circular RNAs
13:10

Use of Alu Element Containing Minigenes to Analyze Circular RNAs

Published on: March 10, 2020

7.4K
Quantification of Circular RNAs Using Digital Droplet PCR
08:39

Quantification of Circular RNAs Using Digital Droplet PCR

Published on: September 16, 2022

3.6K

Related Experiment Videos

Last Updated: Sep 9, 2025

Large-scale Production of Recombinant RNAs on a Circular Scaffold Using a Viroid-derived System in Escherichia coli
10:38

Large-scale Production of Recombinant RNAs on a Circular Scaffold Using a Viroid-derived System in Escherichia coli

Published on: November 30, 2018

9.8K
Use of Alu Element Containing Minigenes to Analyze Circular RNAs
13:10

Use of Alu Element Containing Minigenes to Analyze Circular RNAs

Published on: March 10, 2020

7.4K
Quantification of Circular RNAs Using Digital Droplet PCR
08:39

Quantification of Circular RNAs Using Digital Droplet PCR

Published on: September 16, 2022

3.6K

Area of Science:

  • Biotechnology
  • Molecular Biology
  • RNA Therapeutics

Background:

  • Circular mRNA (mRNA) offers advantages in stability and protein translation duration for mRNA therapy.
  • Efficient in vitro production methods for circular RNA are in high demand.

Purpose of the Study:

  • To develop a versatile and efficient self-circularization strategy for synthesizing circular RNAs.
  • To optimize circularization efficiency, especially for long RNA sequences, using computational methods.

Main Methods:

  • A self-circularization strategy using simple motifs to synthesize circular RNAs.
  • An automated computational program to optimize lock-key structures for enhanced circularization.
  • Leveraging shared sequence and functionality to eliminate purification steps.

Main Results:

  • Achieved robust circularization efficiencies for RNA sequences from dozens to thousands of nucleotides.
  • Demonstrated superior stability and translation efficiency of produced circular RNAs.
  • Enabled sustained protein expression in vitro and in vivo.

Conclusions:

  • The developed method offers a computationally optimized, purification-free approach for scalable circular RNA production.
  • This strategy significantly advances the development of novel RNA therapeutics and mRNA therapy.
  • The method simplifies circular RNA synthesis, enhancing its therapeutic potential.