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FRET Microscopy for Real-time Monitoring of Signaling Events in Live Cells Using Unimolecular Biosensors

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Published on: August 20, 2012

FRET Materials for Biosensing and Bioimaging.

Ruifang Su^1,2,3, Laura Francés-Soriano⁴, P Iyanu Diriwari¹

¹nanoFRET.com, Laboratoire COBRA (UMR6014 & FR3038), Université de Rouen Normandie, CNRS, INSA, Normandie Université, 76000 Rouen, France.

Chemical Reviews

|August 28, 2025

Summary

This summary is machine-generated.

Förster resonance energy transfer (FRET) and nanosurface energy transfer (NSET) biosensing rely on donor-acceptor materials. This review covers diverse FRET/NSET materials, their applications, and properties for biomolecular interaction analysis.

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Area of Science:

Biophysics
Materials Science
Biotechnology

Background:

Förster resonance energy transfer (FRET) and nanosurface energy transfer (NSET) are crucial for analyzing biomolecular interactions.
The development of FRET biosensing systems heavily depends on the materials used for the donor-acceptor pair.

Purpose of the Study:

To review a wide variety of FRET and NSET materials.
To provide examples of FRET/NSET systems and their applications.
To critically discuss the benefits and drawbacks of these FRET/NSET materials.

Main Methods:

Recapitulation of FRET and NSET theory.
Comprehensive review of FRET and NSET materials, including organic dyes, proteins, quantum dots, and gold nanoparticles.
Analysis of material properties and their suitability for sensing applications.

Main Results:

FRET/NSET materials encompass small molecules, nanoscaffolds, and nanomaterials operating across UV, visible, and infrared spectra.
Materials exhibit diverse optical properties like fluorescence and phosphorescence with varying lifetimes.
Gold nanoparticles are highlighted as effective NSET acceptors due to surface plasmon resonance.

Conclusions:

A broad spectrum of FRET/NSET materials is available for biosensing, each with unique advantages and limitations.
The choice of material significantly impacts the performance and applicability of FRET/NSET biosensing systems.
Continued exploration of novel FRET/NSET materials will expand capabilities for in situ, in vitro, and in vivo analyses.