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Updated: Sep 9, 2025

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Published on: October 27, 2011
Biological Role of Replication Factor C Subunit 4 in Cervical Cancer Cell Progression
Seon-Joo Park1, Jae Woong Koh2, Tae-Bum Lee1
1Department of Premedical Science, College of Medicine, Chosun University, Gwangju, Republic of Korea.
Background/Aim:
Cervical cancer, which arises in the cervical epithelial cells, is mainly caused by persistent infection with high-risk human papillomavirus. Replication factor C subunit 4 (RFC4) is instrumental in DNA replication and repair. This study elucidated the role of RFC4 in cervical cancer by in vitro validation.
Materials And Methods:
Quantitative real-time PCR and western blotting were performed to confirm RFC4 mRNA and protein expression levels. Following RFC4 knockdown using small interfering RNA, cervical cancer cells HeLa, ME-180, and SiHa were assessed for cell proliferation, metastatic potential, and cell cycle distribution using MTT, colony formation, migration and invasion assays, and flow cytometry.
Results:
RFC4 mRNA and protein expression was up-regulated in cervical cancer cells. RFC4 knockdown prevented cell proliferation, migration, and invasion in cervical cancer cells. Additionally, RFC4 knockdown induced cell-cycle arrest, inhibiting cell proliferation.
Conclusion:
RFC4 plays a key role in the progression and metastasis of cervical cancer cells and RFC4 may be an important therapeutic target in patients with cervical cancer.
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