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A Microfluidic Chip for the Versatile Chemical Analysis of Single Cells

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Kappa(κ)Chip: a modular microfluidic device for analyte screening using parallelized assays and a multiple shear rate

Jose A Wippold¹, Mark T Kozlowski¹, Joseph La Fiandra²

¹United States Army Combat Capabilities Development Command (DEVCOM) Army Research Laboratory, USA. jose.a.wippold.civ@army.mil.

|August 29, 2025

Summary

Researchers developed a novel microfluidic chip, the kappa(κ)Chip, for efficient screening of bioinspired adhesives. This high-throughput platform accelerates the discovery of strong biological adhesives by enabling 24 simultaneous tests, reducing time and material use.

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Area of Science:

Biomaterials Science
Microfluidics
Synthetic Biology

Background:

Polymers often exhibit low surface energies, hindering adhesive interactions.
Biology offers vast potential for discovering novel adhesive materials through bioinspiration.
High-throughput, low-volume screening methods are crucial for discovering new bioinspired adhesives.

Purpose of the Study:

To develop a novel microfluidic chip, the kappa(κ)Chip, for efficient high-throughput screening of bioinspired adhesives.
To enable rapid evaluation and comparison of multiple adhesive candidates against various substrates.
To facilitate the discovery of strong biological adhesives using a microfluidic platform.

Main Methods:

Development of the kappa(κ)Chip, a parallelized microfluidic device enabling 24 simultaneous adhesion tests.
Utilizing microfabrication, microfluidics, material sciences, synthetic biology, multiphysics simulation, and machine learning (ML).
Evaluating fungal hydrophobin proteins displayed on cell surfaces as a proxy for adhesive properties.

Main Results:

The kappa(κ)Chip significantly enhances testing efficiency, reducing experimental time and reagent consumption.
The platform allows for rapid rank-ordering of potential binding motifs against arbitrary substrates.
Successful evaluation of engineered fungal hydrophobins as potential biological adhesives.

Conclusions:

The kappa(κ)Chip is a powerful tool for accelerating the discovery of novel bioinspired adhesives.
This microfluidic platform integrates multiple scientific disciplines for advanced materials discovery.
The technology has potential applications in cell adhesion studies and marine fouling research.