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ENKD1 Modulates Skin Elasticity Through Microtubule Stability Regulation.

Dan Dong1, Mingzheng Hu1, Xiaofan Wu2

  • 1Department of Genetics and Cell Biology, State Key Laboratory of Medicinal Chemical Biology, College of Life Sciences, Haihe Laboratory of Cell Ecosystem, Nankai University, Tianjin, China.

Cytoskeleton (Hoboken, N.J.)
|August 29, 2025
PubMed
Summary

Enkurin domain-containing protein 1 (ENKD1) is crucial for skin elasticity by stabilizing microtubules in basal keratinocytes. Its absence impairs keratinocyte migration, reducing skin elasticity and function.

Keywords:
cell migrationenkurin domain‐containing protein 1 (ENKD1)keratinocytesmicrotubule stabilityskin elasticity

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Area of Science:

  • Dermatology
  • Cell Biology
  • Molecular Biology

Background:

  • Skin elasticity is vital for skin function.
  • The molecular mechanisms regulating skin elasticity are not fully understood.
  • Microtubule stability plays a role in cellular processes.

Purpose of the Study:

  • To identify key regulators of skin elasticity.
  • To investigate the role of enkurin domain-containing protein 1 (ENKD1) in skin elasticity.
  • To elucidate the molecular mechanisms by which ENKD1 affects skin elasticity.

Main Methods:

  • Utilized Enkd1 knockout mouse models.
  • Examined keratinocyte migration and epidermal elasticity.
  • Investigated the localization and function of ENKD1 in basal keratinocytes.
  • Analyzed the interaction of ENKD1 with microtubules and EB1.

Main Results:

  • Enkd1 knockout mice exhibited reduced epidermal elasticity.
  • ENKD1 was identified as a key regulator of skin elasticity.
  • ENKD1 enhances microtubule stability in basal keratinocytes.
  • ENKD1 interacts with EB1 to modulate microtubule stability.
  • ENKD1 deficiency destabilizes microtubules, impairing keratinocyte migration.

Conclusions:

  • ENKD1 is a pivotal regulator of mammalian epidermal elasticity.
  • ENKD1 modulates skin elasticity by influencing microtubule stability in basal keratinocytes.
  • These findings offer new molecular insights into skin function and elasticity.