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Updated: Sep 9, 2025

DNA-Tethered RNA Polymerase for Programmable In vitro Transcription and Molecular Computation
Published on: December 29, 2021
Self-propagating amplification with a ss-polyadenine probe enables label-free detection of terminal deoxynucleotidyl
Baoqiang Chen1, Haidong Yang1, Zhuqi Sui1
1Jiaxing Key Laboratory of Molecular Recognition and Sensing, College of Biological and Chemical Engineering, Jiaxing University, Jiaxing, 314001, China. jgxu0816@163.com.
Abstract:
We developed a label-free, self-propagating amplification strategy for ultrasensitive terminal deoxynucleotidyl transferase (TdT) detection. The system integrates TdT-triggered poly-T extension, poly-A-primed synthesis, nicking-mediated recycling, and SYBR Green I-based fluorescence output, achieving robust signal amplification and assay performance.
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