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Viral Recombination

Viral Recombination

Cells are sometimes infected by more than one virus at once. When two viruses disassemble to expose their genomes for replication in the same cell, similar regions of their genomes can pair together and exchange sequences in a process called recombination. Alternatively, viruses with segmented genomes can swap segments in a process called reassortment.

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Related Experiment Video

Updated: May 9, 2026

Use of In vivo Imaging to Monitor the Progression of Experimental Mouse Cytomegalovirus Infection in Neonates

Use of In vivo Imaging to Monitor the Progression of Experimental Mouse Cytomegalovirus Infection in Neonates

Published on: July 6, 2013

Visualizing Respiratory Syncytial Virus Replication in Live Mice.

Jean-François Eléouët¹, Marie Galloux², Marie-Anne Rameix-Welti³

¹Unité de Virologie et Immunologie Moléculaires (VIM) UMR0892, INRAE-UVSQ-Université Paris-Saclay, Jouy-en-Josas, France. jean-francois.eleouet@inrae.fr.

Methods in Molecular Biology (Clifton, N.J.)

|August 29, 2025

Summary

Developing better vaccines and treatments for respiratory syncytial virus (RSV) requires effective animal models. Recombinant RSV in mice allows real-time monitoring of infection spread and intensity, improving research into RSV disease.

Keywords:

In vivo model Luciferase Luminescence Mice Preclinical model Replication Visualizing

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Live Imaging and Quantification of Viral Infection in K18 hACE2 Transgenic Mice Using Reporter-Expressing Recombinant SARS-CoV-2

Live Imaging and Quantification of Viral Infection in K18 hACE2 Transgenic Mice Using Reporter-Expressing Recombinant SARS-CoV-2

Published on: November 5, 2021

Area of Science:

Virology
Immunology
Infectious Diseases

Background:

Modeling human respiratory syncytial virus (RSV) infection in vivo is crucial for developing new vaccines and therapies.
Rodent models, particularly mice and cotton rats, are commonly used for RSV infection studies.
Traditional methods like RT-qPCR can estimate viral load but lack real-time monitoring of infection dynamics.

Purpose of the Study:

To highlight the advancement in studying respiratory syncytial virus (RSV) replication in murine models.
To introduce the utility of recombinant RSV expressing firefly luciferase for in vivo studies.

Main Methods:

Utilizing inbred BALB/c mice as a model for respiratory syncytial virus (RSV) infection.
Employing recombinant RSV expressing firefly luciferase to monitor infection in real-time.
Assessing viral load and infection intensity over time within the same animal.

Main Results:

Recombinant RSV allows for non-invasive, longitudinal monitoring of infection spread and intensity in mice.
This method provides a significant improvement over traditional viral load estimation techniques.
Enables detailed analysis of RSV replication dynamics in a living organism.

Conclusions:

Recombinant RSV expressing firefly luciferase offers a powerful tool for studying RSV infection in murine models.
This approach enhances the understanding of RSV pathogenesis and facilitates the evaluation of potential interventions.
The real-time monitoring capability accelerates the search for effective RSV vaccines and antiviral therapies.