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Serial Adaptation of Viruses for Reduced Susceptibility Profiling
Michael R Sirrine1, Richard K Plemper2
1Center for Translational Antiviral Research, Georgia State University Institute for Biomedical Sciences, Atlanta, GA, USA.
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Serial adaptation of viruses to inhibitors enhances the understanding of the mechanism of inhibition, helps to identify physical target sites of compounds, and serves as a test for the genetic barrier to reducing virus susceptibility. Virus adaptations can be carried out in vivo or in tissue culture using sub-inhibitory concentrations of compounds that are increased with each passage of the virus or kept static. Here we describe the method of serially adapting a virus in tissue culture.
