[The isolated living hair cell. A new model for the study of hearing function]

Living, predominantly outer hair cells were prepared from the guinea pig cochlea using a non-enzymatic, microsurgical approach. Viability of hair cells was demonstrated by dye-exclusion as well as by electrophysiogical patch-damp procedures. This allowed to measure a mammalian hair cell potential of -70 mV under direct visualisation. Furthermore, hair cells could be kept under cell culture conditions for 6-9 hrs. Isolated, mammalian cochlea hair cells are a new, useful model to investigate the molecular basis of hearing and hearing disorders located in sensory cells. As example this report shows experimental approaches to analyse membraneous and cytoplasmic functions of hair cells.