The Use of Grating-Coupled Interferometry to Study Inositol Pyrophosphate-Protein Interactions
Kristina Sturm1, Michael Hothorn2
1Structural Plant Biology Laboratory, Department of Plant Sciences, University of Geneva, Geneva, Switzerland.
Methods in Molecular Biology (Clifton, N.J.)
|August 29, 2025
Abstract
None:
Grating-coupled interferometry (GCI) is a biophysical method suitable to studying the binding kinetics of protein-ligand interactions. Here, we describe the application of biotinylated inositol pyrophosphate (PP-InsP) nutrient messengers to characterize PP-InsP-protein interactions using a Creoptix WAVEsystem.


