Related Experiment Video
Updated: Sep 9, 2025
Preparation of Quality Inositol Pyrophosphates
Published on: September 3, 2011
Visualizing and Identifying Inositol Pyrophosphate Isomers in Crystallo
Guangning Zong1, Huanchen Wang2
1Genomic Integrity and Structural Biology Laboratory, National Institute of Environmental Health Sciences, NIH, Research Triangle Park, NC, USA. guangning.zong@nih.gov.
Abstract:
Inositol pyrophosphates (PP-InsPs), which possess functionally significant diphosphate groups, regulate a wide range of biological processes across eukaryotic organisms. The presence and isomeric diversity of PP-InsPs suggest potential biological relevance and enable these molecules to serve as versatile regulators. Identification and characterization of the PP-InsP isomers require specialized approaches. We have successfully utilized X-ray crystallography to directly visualize and identify PP-InsP isomers, thereby informing biological studies and advancing our understanding of their functional roles. Here, we describe detailed methods for identifying PP-InsP isomers in Crystallo.
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