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Updated: Sep 9, 2025

Studying DNA Looping by Single-Molecule FRET
Published on: June 28, 2014
Modelling the melting of DNA oligomers with non-inert dangling ends
Alejandro Soto1, Francesco Mambretti2, Emanuele Locatelli3,4
1Institute of Biological and Chemical Systems - Biological Information Processing, Karlsruhe Institute of Technology, Karlsruhe, Germany.
None:
In this work, we investigate the dependence of the melting temperature of low-valency DNA constructs on the length of non-inert dangling ends, controlling their sequence composition. We compare two computational models to evaluate their effectiveness and limitations in predicting the melting behavior of DNA oligomers (bivalent linkers) and more complex structures (trivalent nanostars), benchmarking the results against experimental spectroscopic data. Our results suggest that the length of non-inert dangling ends has minimal impact on the melting point of the DNA duplex for the duplexes we studied, informing the future design of DNA supramolecular constructs.
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