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Updated: Sep 9, 2025

A Reporter Assay to Analyze Intronic microRNA Maturation in Mammalian Cells
Published on: June 16, 2022
The microRNA miR-243 directs poly(UG) modification of a somatic mRNA in C. elegans
David D Lowe1, Martin Newman2, Gary Ruvkun1,3
1Genetics, Harvard Medical School, Boston, MA, USA.
None:
C. elegans RDE-3 adds poly UG tails to mRNAs targeted for silencing by the dsRNA-initiated RNA interference (RNAi) pathway. RDE-3 can also add p(UG) tails to some endogenous cellular mRNAs. Mechanisms directing RDE-3 to pUGylate specific mRNAs are not understood. Here we show that the miRNA miR-243 directs pUGylation of an intestine-specific mRNA y47h10a.5 , which leads to silencing of the mRNA. The data show that genome-encoded small regulatory RNAs are one mechanism by which RDE-3 can be directed to pUGylate specific mRNAs for gene regulation.
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