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Attachment of Mycoplasma pulmonis to rat and mouse synovial cells cultured in vitro
Abstract:
The attachment of Mycoplasma pulmonis m53 organisms to mouse and rat synovial cells was examined by using the organisms and the synovial cells treated in various ways. M. pulmonis treated with trypsin attached to the synovial cells, but the organisms treated with pronase, formaldehyde, glutaraldehyde, or heat did not. These findings suggest that the sites for binding M. pulmonis to the mouse and rat synovial cells are of polypeptide nature. Treatment of M. pulmonis with sialic acid and treatment of the synovial cell sheets with neuraminidase did not affect the attachment. The synovial cell surface for receptors M. pulmonis organisms would be different from those on respiratory cells or erythrocytes for M. pneumoniae or M. gallisepticum. Even nonviable organisms and M. pulmonis membranes attached to the mouse or rat synovial cells. The nature of the receptor of mouse synovial cells would be different from that of rat cells, since rat cells were affected by treatment with formaldehyde or glutaraldehyde, but mouse cells were not.
Insights
Mycoplasma pulmonis binds to synovial cells via polypeptide sites. Mouse and rat synovial cells differ in Mycoplasma pulmonis receptor characteristics, suggesting distinct binding mechanisms.
Area of Science:
- Microbiology
- Cell Biology
- Immunology
Background:
- Mycoplasma pulmonis is a pathogen that can infect synovial cells.
- Understanding the attachment mechanisms of M. pulmonis is crucial for developing targeted therapies.
Purpose of the Study:
- To investigate the molecular nature of the interaction between Mycoplasma pulmonis and synovial cells from mice and rats.
- To identify the specific binding sites and receptors involved in this attachment process.
Main Methods:
- Treatment of M. pulmonis organisms with various agents (trypsin, pronase, formaldehyde, glutaraldehyde, heat, sialic acid) to identify essential surface components for attachment.
- Treatment of synovial cells with neuraminidase to assess the role of cell surface receptors.
- Examination of attachment using nonviable organisms and isolated M. pulmonis membranes.
Main Results:
- Trypsin treatment of M. pulmonis abolished attachment, indicating a polypeptide nature of the binding sites.
- Pronase, formaldehyde, glutaraldehyde, or heat treatment of M. pulmonis prevented attachment.
- Sialic acid treatment of M. pulmonis and neuraminidase treatment of synovial cells did not affect attachment.
- Nonviable M. pulmonis organisms and membranes also attached to synovial cells.
- Mouse and rat synovial cells exhibited differential responses to formaldehyde and glutaraldehyde treatment, suggesting distinct receptor characteristics.
Conclusions:
- The attachment of Mycoplasma pulmonis to mouse and rat synovial cells is mediated by polypeptide structures on the organism's surface.
- The receptors on mouse and rat synovial cells for M. pulmonis differ, indicating species-specific interactions.
- These findings suggest that M. pulmonis uses different surface receptors compared to other Mycoplasma species that infect respiratory cells or erythrocytes.