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  11. Repression Of Microbial Arsenite Uptake And Methylation By Dissolved Organic Carbon.
  1. Home
  3. Research Domains
  5. Chemical Sciences
  7. Inorganic Chemistry
  9. Inorganic Green Chemistry
  11. Repression Of Microbial Arsenite Uptake And Methylation By Dissolved Organic Carbon.

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Repression of microbial arsenite uptake and methylation by dissolved organic carbon.

Hyun Yoon1, Michael A P Vega1, Jiaxing Wang2

  • 1School of Civil and Environmental Engineering, Cornell University, Ithaca, New York, 14853, United States.

Environmental Science & Technology Letters
|August 29, 2025

View abstract on PubMed

Summary
This summary is machine-generated.

Microbial arsenic methylation in rice paddies is repressed by high carbon substrates like glucose. This carbon catabolite repression (CCR) mechanism affects arsenite uptake via GlpF channels, impacting arsenic biogeochemistry.

Keywords:
arsMarsenicbioavailabilitybiosensor

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Area of Science:

  • Environmental Microbiology
  • Biogeochemistry
  • Arsenic Metabolism

Background:

  • Arsenic methylation is a key microbial process in the arsenic cycle, particularly in rice paddies.
  • Microbial uptake of arsenite is mediated by the aquaglyceroporin GlpF, linking it to organic carbon utilization.

Purpose of the Study:

  • To investigate the hypothesis that carbon substrates repress arsenite uptake and methylation via carbon catabolite repression (CCR).
  • To elucidate the role of GlpF channels and specific carbon substrates in regulating arsenic biotransformation.

Main Methods:

  • Arsenic biosensor assays to measure arsenite uptake.
  • RT-qPCR to analyze the expression of the *glpF* gene.
  • Assessing arsenite methylation by *Arsenicibacter rosenii* with different carbon substrates.
catabolite repression
dissolved organic carbon
glpF
methylation
microbial uptake

Main Results:

  • Arsenite uptake was repressed by glucose and dissolved organic matter (DOM).
  • Increased carbon concentrations led to repression of *glpF* gene expression, reducing arsenite uptake.
  • Arsenite methylation was repressed by upper glycolytic substrates (glucose, xylose, mannose) but not by pyruvate or succinate.

Conclusions:

  • Organic carbon availability, particularly upper glycolytic substrates, significantly impacts microbial arsenic methylation.
  • Carbon catabolite repression (CCR) is a key mechanism regulating arsenite uptake and transformation in rice paddy environments.
  • Findings offer new insights into how organic carbon affects microbial arsenic cycling and biotransformation.