Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Modern Molecular Taxonomy

Modern Molecular Taxonomy

Advancements in molecular biology have revolutionized the identification and characterization of bacteria, with multiple methods leveraging DNA sequencing for enhanced precision. As sequencing technologies improve and costs decline, these approaches are increasingly used in clinical, environmental, and evolutionary studies.Multilocus Sequence Typing (MLST) examines several housekeeping genes, essential chromosomal genes encoding cellular functions, to distinguish strains. Approximately...

Applications of Molecular Taxonomy

Applications of Molecular Taxonomy

Molecular taxonomy has revolutionized the understanding and classification of bacteria, providing precise insights into their diversity, evolutionary relationships, and ecological roles. By utilizing molecular techniques such as DNA sequencing and fingerprinting, researchers have made significant strides in various fields related to bacterial studies.Resolving Taxonomic AmbiguitiesMolecular taxonomy has been instrumental in distinguishing closely related bacterial species initially thought to...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Distal Recirculation of Enteral contents Augmented Mechanically (DREAM) Promotes Intestinal Adaptation and Restores Enterohepatic Signaling in Short Bowel Syndrome.

Gastroenterology·2026

Same author

Exploring role of therapeutic plasma exchange for hepatitis A-related acute liver failure: An Indian multi-center cohort study.

Indian journal of gastroenterology : official journal of the Indian Society of Gastroenterology·2026

Same author

Chronic in utero oxycodone exposure alters placental small EV proteome and fetal cardiomyopathy-linked pathways.

Extracellular vesicles and circulating nucleic acids·2026

Same author

Peptide-Based Probe Targeting Angiotensin Type‑1 Receptor Identifies Pancreatic Ductal Adenocarcinoma Using Multispectral Optoacoustic Tomography.

Chemical & biomedical imaging·2026

Same author

Viscous energy dissipation on hemolysis across various flow regimes using a high-shear device.

Medical engineering & physics·2026

Same author

Indian National Association for Study of the Liver Guidance Document on Difficult to Treat Autoimmune Hepatitis.

Journal of clinical and experimental hepatology·2026

Same journal

Divergent Microbiota Dynamics along the Coastal Marine Ecosystem of Puerto Rico.

Microbiology research·2024

Same journal

Omics of an Enigmatic Marine Amoeba Uncovers Unprecedented Gene Trafficking from Giant Viruses and Provides Insights into Its Complex Life Cycle.

Microbiology research·2023

Same journal

Molecular epidemiology of GB type C virus among individuals exposed to hepatitis C virus in Cameroon.

Microbiology research·2021

Same journal

Evaluation of an enzyme-linked immunosorbent assay using purified, deglycosylated histoplasmin for different clinical manifestations of histoplasmosis.

Microbiology research·2011

See all related articles

Search research articles

Related Experiment Video

Updated: Sep 9, 2025

Microbiota Analysis Using Two-step PCR and Next-generation 16S rRNA Gene Sequencing

Microbiota Analysis Using Two-step PCR and Next-generation 16S rRNA Gene Sequencing

Published on: October 15, 2019

Bioinformatic Methodologies in Assessing Gut Microbiota.

James Douglas Fox¹, Austin Sims¹, Morgan Ross¹

¹Department of Pediatrics, Saint Louis University, St. Louis, MO 63103, USA.

Microbiology Research

|August 29, 2025

Summary

This summary is machine-generated.

Bioinformatic methods analyze gut microbiota using sequencing technologies like 16S rRNA and whole-genome sequencing. This review compares these methods to understand the gut microbiome's role in health and disease.

Keywords:

16S rRNA sequencing bioinformatics gastrointestinal gut microbiota intestinal microbiology microbiome shotgun sequencing whole-genome sequencing

More Related Videos

Analysis of Interactions between Endobiotics and Human Gut Microbiota Using In Vitro Bath Fermentation Systems

Analysis of Interactions between Endobiotics and Human Gut Microbiota Using In Vitro Bath Fermentation Systems

Published on: August 23, 2019

A Method to Assess Bacteriocin Effects on the Gut Microbiota of Mice

A Method to Assess Bacteriocin Effects on the Gut Microbiota of Mice

Published on: July 25, 2017

Related Experiment Videos

Last Updated: Sep 9, 2025

Microbiota Analysis Using Two-step PCR and Next-generation 16S rRNA Gene Sequencing

Microbiota Analysis Using Two-step PCR and Next-generation 16S rRNA Gene Sequencing

Published on: October 15, 2019

Analysis of Interactions between Endobiotics and Human Gut Microbiota Using In Vitro Bath Fermentation Systems

Analysis of Interactions between Endobiotics and Human Gut Microbiota Using In Vitro Bath Fermentation Systems

Published on: August 23, 2019

A Method to Assess Bacteriocin Effects on the Gut Microbiota of Mice

A Method to Assess Bacteriocin Effects on the Gut Microbiota of Mice

Published on: July 25, 2017

Area of Science:

Microbiology
Bioinformatics
Genomics

Background:

Gut microbiota analysis is crucial for understanding human health and disease.
Bioinformatic tools are essential for interpreting complex microbial community data.
High-throughput sequencing generates vast datasets for microbiome research.

Purpose of the Study:

To compare 16S rRNA gene sequencing and whole-genome sequencing methodologies.
To elucidate the differences in utilization and potential of these sequencing approaches.
To enhance understanding of the gut microbiome's role in human physiology and pathology.

Main Methods:

Review of bioinformatic methodologies for gut microbiota assessment.
Analysis of high-throughput sequencing technologies (16S rRNA, metagenomics, whole-genome).
Discussion of bioinformatics tools, algorithms, and statistical models for data interpretation.

Main Results:

16S rRNA sequencing provides microbial diversity and taxonomic information.
Whole-genome sequencing offers deeper insights into functional potential but is more costly.
Bioinformatics enables identification of microbial taxa and their roles.

Conclusions:

Bioinformatic approaches are vital for deciphering gut microbiome complexity.
Understanding sequencing method differences aids in microbiome research and therapeutic development.
Optimizing microbiome analysis is key to future health interventions.