Related Experiment Video
Updated: Sep 9, 2025

A Customizable Approach for the Enzymatic Production and Purification of Diterpenoid Natural Products
Published on: October 4, 2019
Euphebranes A-G: higher diterpenoids from the radix of Euphorbia ebracteolata
Shu-Qi Wu1, Lei-Ming Wu1, Jia-Qian Chen1
1School of Pharmaceutical Sciences, Sun Yat-sen University, Guangzhou, 510006, People's Republic of China.
Abstract:
Phytochemical investigation of roots of Euphorbia ebracteolata Hayata led to ten higher diterpenoids including seven undescribed ones (1-7) and three analogues (8-10), accompanied by four derivatives (7a and 9a-9c) obtained via chemical correlation. Notably, compound 1 represents a rare dinor-ent-isopimarane diterpenoid. Their structures were fully characterized using a combination of spectroscopic, ECD, chemical, and single-crystal X-ray diffraction means. Cytotoxicity screening suggested that the oxidized derivative of 7 (7a) displayed the most potent activity against non-small-cell lung cancer (NSCLC) cell line HCC827, comparable to the positive control, cisplatin. Mechanistic study revealed that 7a could arrest cell cycle at G1/S phase and induce apoptosis.
Related Concept Videos
Structure and Nomenclature of Epoxides
Crown Ethers
Structure and Nomenclature of Ethers
Ethers are organic compounds with an ether functional group which is characterized by an oxygen atom connected to two — identical or different — alkyl, aryl, or vinyl groups. The C–O–C linkage in dimethyl ether — the simplest ether — has an approximately tetrahedral bond angle of 110.3 degrees. The oxygen atom is sp3- hybridized, with the C–O distance being about 140 pm.
Classification of Ethers
Based on their attached substituent...
Structure of Conjugated Dienes
Conjugated dienes are compounds characterized by the presence of alternating double and single bonds. In a conjugated system like 1,3-butadiene, the unhybridized 2p orbital on each carbon overlaps continuously, allowing the π electrons to be delocalized across the entire molecule. In contrast, this type of overlap does not occur in cumulated and isolated dienes, such as 2,3-pentadiene and 1,4-pentadiene, respectively. Instead, the π electrons remain localized between the double...
Diels–Alder Reaction Forming Bridged Bicyclic Products: Stereochemistry
UV–Vis Spectroscopy: Woodward–Fieser Rules

