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Thin-layer Chromatographic TLC Separations and Bioassays of Plant Extracts to Identify Antimicrobial Compounds
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Thin-layer Chromatographic TLC Separations and Bioassays of Plant Extracts to Identify Antimicrobial Compounds

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Thin Layer Chromatography Bioautography Scanning Electron Microscopy (TLCBA SEM) method for antimicrobial fraction

Anna du Toit1, Chaitali Dekiwadia1, Lisa Cornell1

  • 1School of Science & Microscopy & Microanalysis Facility, Royal Melbourne Institute of Technology University, Melbourne, Australia.

Journal of Microbiological Methods
|August 29, 2025
PubMed

Keywords:
AntibacterialScanning electron microscopyStructure damageTargetThin layer chromatography-bioautography

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Thin-Layer Chromatography (TLC): Overview01:11

Thin-Layer Chromatography (TLC): Overview

Thin-layer chromatography (TLC) is a chromatography technique that separates compounds based on their polarity. TLC typically uses polar silica gel, a form of silicon dioxide, as the stationary phase. The silica gel contains hydroxyl (OH) groups on its surface, which form hydrogen bonds with polar compounds, influencing their adhesion to the stationary phase.
To begin the analysis, a mixture of compounds is spotted on the starting line on the TLC plate using a thin capillary. The bottom of the...