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CRISPR tools for T cells: targeting the genome, epigenome, and transcriptome

Tassilo L A Wachsmann1, Lei S Qi2

  • 1Department of Bioengineering, Stanford University, Stanford, CA, USA.

Trends in Cancer
|August 29, 2025
PubMed

Insights

CRISPR gene editing offers new solutions for T cell therapy challenges in cancer treatment, improving efficacy and accessibility for solid tumors. This technology enhances T cells for next-generation cancer therapies.

Area of Science:

  • Immunology
  • Genetics
  • Biotechnology

Background:

  • T cell therapy shows promise for cancers but faces challenges like response duration, antigen escape, and solid tumor treatment.
  • Current limitations hinder T cell therapy's broad application and effectiveness.

Purpose of the Study:

  • To review advancements in CRISPR technologies for T cell engineering.
  • To explore how CRISPR can overcome current T cell therapy limitations.
  • To highlight future directions for CRISPR-based cellular therapy.

Main Methods:

  • Review of recent technological advancements in CRISPR systems.
  • Analysis of CRISPR applications in T cell engineering.
  • Discussion of emerging applications and limitations.

Main Results:

  • CRISPR technologies have evolved beyond gene knockout to sophisticated genome, epigenome, and transcriptome engineering.
  • CRISPR offers potential solutions for T cell therapy's challenges, including efficacy and solid tumor treatment.

Conclusions:

  • CRISPR technology is pivotal for advancing T cell therapy.
  • Future development of CRISPR tools will address current limitations and expand therapeutic potential.
  • CRISPR-engineered T cells represent the next generation of cellular cancer therapy.
Keywords:
CRISPR-CasT cell receptor (TCR)chimeric antigen receptor (CAR)epigenome engineeringtranscriptome engineeringtumor-infiltrating lymphocyte (TIL)

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