Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Epigenetic Regulation01:37

Epigenetic Regulation

3.1K
Epigenetic changes alter the physical structure of the DNA without changing the genetic sequence and often regulate whether genes are turned on or off. This regulation ensures that each cell produces only proteins necessary for its function. For example, proteins that promote bone growth are not produced in muscle cells. Epigenetic mechanisms play an essential role in healthy development. Conversely, precisely regulated epigenetic mechanisms are disrupted in diseases like cancer.
X-chromosome...
3.1K
Methods of Nuclear Reprogramming01:24

Methods of Nuclear Reprogramming

1.9K
Nuclear reprogramming is a process of transforming one cell type into an unrelated cell type by epigenetic changes that alter the cell’s original gene expression pattern. Such epigenetic changes force cells to express a different set of genes, which play a significant role in inducing transformation into other cell types. Nuclear reprogramming offers applications in reproductive cloning for livestock propagation and regenerative medicine — developing patient-specific cells for...
1.9K
Chromatin Modification in iPS Cells01:32

Chromatin Modification in iPS Cells

1.9K
Chromatin modification alters gene expression; therefore, scientists can add histone-modifying enzymes, histone variants, and chromatin remodeling complexes to somatic cells to aid reprogramming into pluripotent stem (iPS) cells.
Compact chromatin makes reprogramming difficult. Enzymes, such as histone demethylases and acetyltransferases, are often added during reprogramming to loosen the chromatin, making the DNA more accessible to transcription factors. Molecules that inhibit histone...
1.9K
Regulation of Expression at Multiple Steps01:23

Regulation of Expression at Multiple Steps

994
The gene expression in cells is regulated at different stages: (i) transcription, (ii) RNA processing, (iii) RNA localization, and (iv) translation. Transcriptional regulation is mediated by regulatory proteins such as transcription factors, activators, or repressors—these control gene expression by initiating or inhibiting the transcription of genes. Once a precursor or pre-mRNA is produced, it undergoes post-transcriptional modification, including 5' capping, splicing, and the...
994
Somatic to iPS Cell Reprogramming01:29

Somatic to iPS Cell Reprogramming

2.3K
Reprogramming alters the gene expression in somatic cells, transforming them into induced pluripotent stem (iPS) cells over several generations. Scientists can reprogram cells by introducing genes for four transcription factors—Oct4, Sox2, Klf4, and c-Myc (OSKM) by viral or non-viral methods. These factors are also known as Yamanaka factors after Shinya Yamanaka, who first generated iPS cells using mouse skin cells. Yamanaka was awarded the Nobel Prize in Physiology or Medicine in 2012...
2.3K
Regulation of Expression Occurs at Multiple Steps02:24

Regulation of Expression Occurs at Multiple Steps

3.1K
3.1K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Synthesis of bisindole scaffolds by palladium-catalyzed allylic alkylation of <i>N</i>-aminoindole with <i>ortho</i>-iodoaryl allenes.

Organic & biomolecular chemistry·2026
Same author

FTO Controls Endometrial Receptivity and Embryo Implantation through Regulating m<sup>6</sup>A and H3K27me3.

Communications biology·2026
Same author

Ythdf2/Setd1b regulatory axis is essential for cerebellar development through regulating epigenetic reprogramming.

Molecular psychiatry·2026
Same author

METTL3 Affects Endometrial Receptivity Through Modulation of P62-Mediated Autophagic Flux.

FASEB journal : official publication of the Federation of American Societies for Experimental Biology·2025
Same author

RNA-m5C regulatory atlas of human fetal tissues uncover the activities of Nsun2/Jarid2/Alyref axis.

Journal of advanced research·2025
Same author

An overview of RecQ helicases and related diseases.

Aging·2025

Related Experiment Video

Updated: Sep 9, 2025

Characterizing RNA Modifications in Single Neurons Using Mass Spectrometry
08:45

Characterizing RNA Modifications in Single Neurons Using Mass Spectrometry

Published on: April 21, 2022

2.5K

Fto-mediated m6A modification is essential for cerebellar development through regulating epigenetic reprogramming.

Jing Jiang1, Ming Zhang1, Wenjuan Xia1

  • 1State Key Laboratory of Reproductive Medicine and Offspring Health (Suzhou Centre), Suzhou Municipal Hospital, Gusu School, Suzhou Affiliated Hospital of Nanjing Medical University, Nanjing Medical University, Suzhou, 215002, China.

Journal of Biomedical Science
|August 29, 2025
PubMed
Summary

Loss of the Fto gene in mice caused cerebellar ataxia due to altered epitranscriptomic regulation. This study reveals Fto

Keywords:
FtoKat8Cerebellar developmentH4K16acKeywordsm6A modification

More Related Videos

A Method for Measuring RNA N6-methyladenosine Modifications in Cells and Tissues
08:56

A Method for Measuring RNA N6-methyladenosine Modifications in Cells and Tissues

Published on: December 5, 2016

11.0K
Methylated RNA Immunoprecipitation Assay to Study m5C Modification in Arabidopsis
08:50

Methylated RNA Immunoprecipitation Assay to Study m5C Modification in Arabidopsis

Published on: May 14, 2020

6.8K

Related Experiment Videos

Last Updated: Sep 9, 2025

Characterizing RNA Modifications in Single Neurons Using Mass Spectrometry
08:45

Characterizing RNA Modifications in Single Neurons Using Mass Spectrometry

Published on: April 21, 2022

2.5K
A Method for Measuring RNA N6-methyladenosine Modifications in Cells and Tissues
08:56

A Method for Measuring RNA N6-methyladenosine Modifications in Cells and Tissues

Published on: December 5, 2016

11.0K
Methylated RNA Immunoprecipitation Assay to Study m5C Modification in Arabidopsis
08:50

Methylated RNA Immunoprecipitation Assay to Study m5C Modification in Arabidopsis

Published on: May 14, 2020

6.8K

Area of Science:

  • Neuroscience
  • Epigenetics
  • Molecular Biology

Background:

  • Epitranscriptomic regulation, particularly m6A methylation, is crucial for cerebellar development and function.
  • The specific role of the RNA demethylase Fto in the cerebellum remains unclear.

Purpose of the Study:

  • To investigate the function of Fto in cerebellar development and function using a knockout mouse model.
  • To elucidate the molecular mechanisms underlying Fto's role in cerebellar development.

Main Methods:

  • Generated Fto knockout (FtoKO) mice for phenotypic analysis.
  • Assessed cerebellar function via behavioral tests and Nissl staining.
  • Utilized molecular techniques including immunofluorescence, m6A-RIP-seq, ATAC-seq, CUT&Tag-seq, and Co-IP to analyze gene expression, m6A levels, and chromatin accessibility.

Main Results:

  • FtoKO mice displayed cerebellar ataxia with tremors and abnormal gait.
  • Reduced FTO expression led to altered expression of neuronal development and self-renewal genes.
  • Mechanistically, Fto loss resulted in Kat8 upregulation, increased m6A levels, and altered H4K16ac modification, impacting chromatin accessibility.

Conclusions:

  • Fto plays a critical role in cerebellar development.
  • Fto deficiency disrupts cerebellar function through m6A-dependent regulation of Kat8 and chromatin accessibility.
  • These findings highlight the importance of epitranscriptomic regulation in neurodevelopmental processes.