Simultaneous determination of some basic dyes using p-tetranitrocalix[4]arene-appended silica-based HPLC column.

Area of Science:

• Analytical Chemistry
• Materials Science

Background:

• Development of advanced stationary phases is crucial for improving chromatographic separations.
• Calixarene-based materials offer unique properties for chromatographic applications.

Purpose of the Study:

• To synthesize and characterize a p-tetranitrocalix[4]arene-appended silica-based material.
• To evaluate its application as a stationary phase for the simultaneous separation of basic dyes using high-performance liquid chromatography (HPLC).

Main Methods:

• Synthesis and characterization of the functionalized silica material.
• HPLC analysis for separating methyl violet, methyl green, and methylene blue.
• Investigation of mobile phase pH effects on separation.
• Method validation including linearity, accuracy, precision, and limits of detection/quantification.

Main Results:

• The p-tetranitrocalix[4]arene-appended silica-based material demonstrated effective separation of basic dyes.
• Optimal separation was achieved by adjusting the mobile phase pH.
• The method exhibited good linearity, accuracy, and precision with low limits of detection and quantification.
• Separation efficiency was compared to a standard octadecylsilane column.

Conclusions:

• The developed p-tetranitrocalix[4]arene-appended silica stationary phase is a promising material for the simultaneous separation of basic dyes in HPLC.
• The study provides a validated method for dye analysis with potential applications in environmental monitoring and quality control.