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Updated: Sep 9, 2025

A Generalized Method for Determining Free Soluble Phenolic Acid Composition and Antioxidant Capacity of Cereals and Legumes
Published on: June 10, 2022
Evaluating the antioxidant capacity of polyphenols with an off-on fluorescence probe and the mechanism study
Linying Cao1,2, Hui Yu1,2, Shijun Shao1
1Key Laboratory of Chemistry of Northwestern Plant Resources and Key Laboratory for Natural Medicine of Gansu Province, Lanzhou institute of Chemical Physics, Chinese Academy of Sciences, Lanzhou 730000, P. R. China. guoyong@licp.cas.cn.
Abstract:
A spin-labeled fluorescence probe (RNO˙) was employed to evaluate the antioxidant capacity of polyphenols. The hydrogen-transfer mechanism was proposed by kinetic studies using fluorescence spectroscopy, and the product was confirmed to be RNOH by HPLC-MS with little side products. The HPLC-UV/vis method devoted to the simultaneous monitoring of the RNO˙ and RNOH at 556 nm was developed, which can distinguish the antioxidant capacity of polyphenols with similar structure. The method has the potential to be used to screen antioxidants when modifying or optimizing natural antioxidants.

