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Updated: Sep 9, 2025

Simultaneous Measurement of Superoxide/Hydrogen Peroxide and NADH Production by Flavin-containing Mitochondrial Dehydrogenases
Published on: February 24, 2018
Amperometric determination of NADH based on a poly-Ni(II)-curcumin composite film modified glassy carbon electrode
Xiaoqian Ouyang1, Yaping Ding1, Xi Ba1
1Department of Chemistry, Shanghai University, Shanghai 200444, P R China. wdingyp@sina.com.
Abstract:
A poly-Ni(II)-curcumin (curcumin: 1,7-bis[4-hydroxy-3-methoxyphenyl]-1,6-heptadiene-3,5-dione) composite film modified electrode was prepared for amperometric determination of β-nicotinamide adenine dinucleotide (NADH). Scanning electron microscopy and Energy Dispersive X-ray spectroscopy were used to examine the morphology and interface property of the Ni(II)-curcumin film. Under optimum conditions, the amperometric detection of NADH provided a wide linear detection range (0.3-300 μM), high sensitivity (230.02 μA mM-1 cm-2) and low limit of detection (LOD = 0.18 μM, S/N = 3). Moreover, the modified electrode showed good stability and reproducibility, indicating that it can improve the analysis signal of NADH in a very convenient way.
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