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Updated: Sep 9, 2025

Measuring Gene Expression in Bombarded Barley Aleurone Layers with Increased Throughput
Published on: March 30, 2018
The transcription factor ANAC053 confers aluminum tolerance via regulation of a cell wall modifying gene
Jing Huang1,2, Jia Tong He1,3,4, Chuan Jin Shan1,4,5
1State Key Laboratory of Soil and Sustainable Agriculture, Institute of Soil Science, Chinese Academy of Science, Nanjing 210008, China.
Abstract:
Cell wall modification alters plant resistance to aluminum (Al), although the mechanisms remain unclear. In this study, we identified the No Apical Meristem (NAM), Arabidopsis Transcription Factor 1/2 (ATAF1/2) and Cup-shaped Cotyledon 2 (CUC2) (NAC) transcription factor (TF) ANAC053 as crucial for Al resistance in Arabidopsis. (Arabidopsis thaliana) Al caused a concurrent increase in ANAC053 transcript and protein accumulation, and anac053 mutants were sensitive to Al, whereas transgenic lines overexpressing ANAC053 exhibited reduced Al sensitivity. Additionally, the anac053 mutants possessed elevated xyloglucan levels, causing higher cell wall Al fixation capacity and indicating that ANAC053 affects Al accumulation in the roots. We revealed that ANAC053 directly regulates the expression of XYLOGLUCAN ENDOTRANSGLUCOSYLASE HYDROLASE 15 (XTH15), which is primarily responsible for xyloglucan remodeling. In conclusion, our findings show that ANAC053 directly regulates cell wall modification-associated genes that are necessary for root growth under Al stress and that plant Al tolerance is dictated by the capacity of ANAC053 to sequester Al within the root cell wall.
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Transcription
Transcription is the process of synthesizing RNA from a DNA sequence by RNA polymerase. It is the first step in producing a protein from a gene sequence. Additionally, many other proteins and regulatory sequences are involved in the proper synthesis of messenger RNA (mRNA). Regulation of transcription is responsible for the differentiation of all the different types of cells and often for the proper cellular response to environmental signals.
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There are several different mechanisms used to attenuate transcription. In ribosome mediated...
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