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Updated: May 4, 2026
06:24
Generic Protocol for Optimization of Heterologous Protein Production Using Automated Microbioreactor Technology
Published on: December 15, 2017
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Process mapping and optimization study of CHO cell cultures for mAb production using Ambr® 250 high-throughput
A Bordoloi1,2, F Talebnia Rowshan3,4
1Department of Sustainable Chemical, Biological and Materials Engineering, University of Oklahoma, Norman, 73019, USA.
Bioprocess and Biosystems Engineering
|August 30, 2025
Summary
Optimizing Chinese hamster ovary cell culture with Ambr®250 bioreactors accelerated monoclonal antibody (mAb) development. Key parameters like seeding density and feeding rate were refined, significantly boosting mAb titer and reducing timelines.
Area of Science:
- Biotechnology
- Bioprocess Engineering
- Mammalian Cell Culture
Background:
- Accelerating monoclonal antibody (mAb) development is crucial for timely patient access to therapies.
- Chinese Hamster Ovary (CHO) cells are the primary host for mAb production.
- High-throughput (HTP) bioreactor systems offer precise control for process optimization.
Purpose of the Study:
- To optimize critical process parameters (CPPs) for enhanced mAb production in CHO cells.
- To leverage the Ambr®250 HTP bioreactor system for efficient process development.
- To establish a framework for accelerated early-phase bioprocess development.
Main Methods:
- Systematic mapping approach to identify CPPs.
- Central Composite Design (CCD) in Ambr®250 bioreactors.
- Response surface methodology for parameter optimization.
Main Results:
- Identified significant impacts of seeding density (SD) and feeding rate (FR) on mAb titer.
- Optimal conditions determined: SD > 1x10^6 cells/mL and FR > 2% Vc/day.
- Achieved higher mAb titers up to 5 g/L with maintained cell viability and productivity.
Conclusions:
- Optimized operating ranges for SD and FR enhance mAb production efficacy.
- The Ambr®250 platform facilitates accelerated process development and scalable manufacturing.
- Improved productivity and cost reduction facilitate patient access to biologics.
Keywords:
Ambr®250CHO cellsHigh-throughput bioreactorMonoclonal antibodies (mAbs)Process mappingRSM optimizationMore Related Videos
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