Quanzhenyiqitang and its polysaccharides alleviate chronic obstructive pulmonary disease inflammatory injury through
Shiting Dai1, Jiamin Zeng2, Hongxia Ke1
1Institute of Integration of Traditional and Western Medicine, Guangzhou Medical University, Guangzhou, China; State Key Laboratory of Respiratory Disease, National Clinical Research Center for Respiratory Disease, Guangzhou Institute of Respiratory Health, The First Affiliated Hospital of Guangzhou Medical University, Guangzhou Medical University, Guangzhou, Guangdong, China.
Ethnopharmacological Relevance:
Quanzhenyiqitang (QZYQT) is a classic decoction used for treating chronic obstructive pulmonary disease (COPD), with Quanzhenyiqitang polysaccharides (QZYQTP) identified as the active component. Both have demonstrated effectiveness in treating COPD in clinical and experimental studies. However, the active components and potential mechanisms in treating COPD remain incompletely understood.
Aim Of The Study:
This study aims to identify the efficacious active ingredients, potential therapeutic targets, and underlying mechanisms of QZYQT in treating COPD.
Materials And Methods:
The serum chemical constituents of QZYQT-treated rats were analyzed. Potential targets were subsequently predicted using network pharmacology and were preliminarily validated using molecular docking analysis. These predictions were then experimentally validated. In cell experiments, cell number and morphological changes were observed, and Real-time quantitative PCR was used to detect related inflammatory factors. Immunofluorescence (IF) and Western blotting (WB) were employed to investigate the specific mechanisms of QZYQT and QZYQTP (QZYQT(P)) in treating COPD. In animal experiments, the efficacy of QZYQT(P) was assessed by measuring mouse body weight, pulmonary function, lung tissue pathology, and the expression of lung inflammatory factors. Correlation analysis was performed to evaluate the relationships between lung function metrics, histological findings, and inflammatory cytokine levels. Immunohistochemical and WB analyses were used to clarify the specific mechanisms of QZYQT(P) in treating COPD.
Results:
Network pharmacology and molecular docking analyses indicated that the Toll-like receptor and MAPK signaling pathways likely play a crucial role in the therapeutic effects of QZYQT on COPD. QZYQT(P) could effectively improve lung function, attenuate emphysema, and alleviate airway remodeling, inflammatory infiltration, and collagen deposition in the lungs of COPD model mice. QZYQT(P) also decreased the expression of pro-inflammatory cytokines, such as tumor necrosis factor-α, interleukin-6 (IL-6), and IL-8 in cells and mice to varying degrees. Correlation analysis revealed that lung function metrics correlated with histological findings but not with inflammatory cytokine levels. The IF and IHC experiments indicated that QZYQT(P) decreased TLR4 expression in both cells and mice. WB analysis showed that QZYQT(P) could decrease the levels of TLR4, MyD88, p-JNK, p-p38, and p-c-Jun proteins in cells and mice to varying degrees. Notably, QZYQT(P) decreased p-ERK1/2 levels in mice but had little effect on cells. This discrepancy may be due to differences in models, microenvironments, pharmacokinetics, and drug metabolism.
Conclusions:
QZYQT(P) alleviated inflammatory responses in COPD by modulating the TLR4/MyD88/MAPK signaling pathway, providing a foundation for further clinical treatment.
