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Updated: Sep 9, 2025

Polysome Fractionation and Analysis of Mammalian Translatomes on a Genome-wide Scale
Published on: May 17, 2014
Stage-specific requirement for METTL3-dependent m6A epitranscriptomic regulation during myogenesis
Ye-Ya Tan1, Yang-Wen Ou2, Qin Zuo3
1GMU-GIBH Joint School of Life Science, The Guangdong-Hong Kong-Macao Joint Laboratory for Cell Fate Regulation and Diseases, Guangzhou Medical University, Guangzhou, China.
None:
The regulatory role of N6-methyladenosine (m6A) modification in skeletal muscle myogenesis and muscle homeostasis remains poorly characterized, particularly regarding the functional significance of methyltransferase-like 3 (METTL3), the catalytic subunit of the m6A methyltransferase complex (MTC), in myogenic regulation. Through systematic investigation of m6A epitranscriptomic remodeling during myogenesis, we demonstrate that METTL3-mediated m6As orchestrates myoblast fusion processes in both differentiation and regeneration contexts. Notably, we observed marked induction of Mettl3 expression post-injury, accompanied by substantial transcriptomic alterations in myogenesis-related pathways. High-resolution m6A mapping revealed distinct dynamic patterns of METTL3-regulated m6As during differentiation, exhibiting dichotomous regulation across target transcripts. Mechanistically, we identified myogenic fusion factors Mymx and Mymk as direct targets of METTL3, showing concomitant upregulation of both transcript abundance and m6A deposition during myogenesis. This study provides comprehensive multi-omics resources delineating the mechanistic landscape of METTL3-regulated m6As in myogenic programming, establishing METTL3 as a critical regulatory node governing myoblast fusion dynamic.
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