Light-addressable potentiometric sensors modified with mesoporous silica nanoparticles for label-free detection of...

Adenosine (AD) is integral to numerous physiological processes, thereby necessitating its sensitive and selective detection for clinical diagnostic applications. This study introduces an innovative aptasensor utilizing a light-addressable potentiometric sensor (LAPS) for the accurate detection of AD. The LAPS platform capitalizes on its light-addressable properties and stable potentiometric performance to monitor potential variations resulting from the specific interaction between AD and its corresponding aptamer (AD-Apt). To improve sensitivity, mesoporous silica nanoparticles (MSN) were utilized to modify the surface of the LAPS chip, which provided a substantial surface area for aptamer immobilization and enhanced binding efficiency. The aptasensor achieved an impressive detection limit of 0.01 nM, with a wide linear detection range from 0.05 nM to 100 nM. Furthermore, it exhibited excellent selectivity against potential interferents, high reproducibility, and robust stability across multiple measurements. The aptasensor's effectiveness was further corroborated through the detection of AD in human serum samples, underscoring its practical relevance in clinical environments. The electrostatic detection mechanism at the apt-LAPS interface offers a label-free and highly efficient approach for AD sensing. Given its exceptional performance and simplicity, this aptasensor represents a promising platform for early disease diagnosis and biomedical research.