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Updated: May 7, 2026

Nitropeptide Profiling and Identification Illustrated by Angiotensin II

Nitropeptide Profiling and Identification Illustrated by Angiotensin II

Published on: June 16, 2019

Identification of arginylated N-termini with specific reagents.

Jasper Eising¹, Nico Dissmeyer¹

¹Department of Plant Physiology and Protein Metabolism Laboratory, University of Osnabruck, Osnabruck, Germany; CellNanOs-Center of Cellular Nanoanalytics, University of Osnabruck, Osnabruck, Germany; Faculty of Biology, University of Osnabruck, Osnabruck, Germany.

Methods in Enzymology

|August 31, 2025

Summary

This summary is machine-generated.

N-terminal arginylation modifies proteins, impacting their stability and function. Identifying these arginylated proteins is crucial for research and therapeutic applications, with specific antibodies offering a key detection method.

Keywords:

Antibodies Autophagy N-degron pathway N-terminal arginylation Protein degradation R-catcher

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Last Updated: May 7, 2026

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Area of Science:

Biochemistry
Molecular Biology
Cellular Biology

Background:

N-terminal arginylation is a crucial posttranslational modification impacting protein stability and cellular homeostasis.
Arginylated N-termini can function as N-degrons, targeting proteins for degradation via the proteasome or autophagic-lysosomal system (ALS).

Purpose of the Study:

To explore methods for identifying N-terminally arginylated proteins.
To highlight the significance of arginyl-transferases (Ates) and their substrates in biological research and therapeutic strategies.

Main Methods:

Focus on the development and application of specific antibodies.
Antibodies target N-terminally modified proteins for detection and analysis.

Main Results:

Identification of N-terminally arginylated proteins is essential for understanding protein regulation.
Specific antibodies provide a targeted approach for identifying these modified proteins.

Conclusions:

N-terminal arginylation plays a vital role in protein fate and cellular regulation.
The identification of arginylated substrates using specific antibodies is of high interest across various research fields, including human and plant biology.