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Related Concept Videos

CRISPR01:59

CRISPR

52.9K
Genome editing technologies allow scientists to modify an organism’s DNA via the addition, removal, or rearrangement of genetic material at specific genomic locations. These types of techniques could potentially be used to cure genetic disorders such as hemophilia and sickle cell anemia. One popular and widely used DNA-editing research tool that could lead to safe and effective cures for genetic disorders is the CRISPR-Cas9 system. CRISPR-Cas9 stands for Clustered Regularly Interspaced...
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CRISPR/Cas9 Genome Editing01:28

CRISPR/Cas9 Genome Editing

211
The CRISPR-Cas system serves as a bacterial defense mechanism against invading genetic elements such as viruses and plasmids, forming the foundation for its adaptation as a powerful genome-editing tool. Originally discovered in prokaryotes, this system has been repurposed to revolutionize genetic engineering across a wide range of organisms, including plants, animals, and humans. The core component, Cas9, is an endonuclease derived from Streptococcus pyogenes, capable of introducing...
211
CRISPR and crRNAs02:53

CRISPR and crRNAs

17.3K
Bacteria and archaea are susceptible to viral infections just like eukaryotes; therefore, they have developed a unique adaptive immune system to protect themselves. Clustered regularly interspaced short palindromic repeats and CRISPR-associated proteins (CRISPR-Cas) are present in more than 45% of known bacteria and 90% of known archaea.
The CRISPR-Cas system stores a copy of foreign DNA in the host genome and uses it to identify the foreign DNA upon reinfection. CRISPR-Cas has three different...
17.3K
Homologous Recombination02:31

Homologous Recombination

51.5K
The basic reaction of homologous recombination (HR) involves two chromatids that contain DNA sequences sharing a significant stretch of identity. One of these sequences uses a strand from another as a template to synthesize DNA in an enzyme-catalyzed reaction. The final product is a novel amalgamation of the two substrates. To ensure an accurate recombination of sequences, HR is restricted to the S and G2 phases of the cell cycle. At these stages, the DNA has been replicated already and the...
51.5K
What is Genetic Engineering?00:49

What is Genetic Engineering?

75.4K
Overview
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Conservative Site-specific Recombination and Phase Variation02:53

Conservative Site-specific Recombination and Phase Variation

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Because the DNA segments are cut and reorganized in a direction-specific manner, site-specific recombination has emerged as an efficient genetic engineering technique. Flippase and Cyclization recombinases or Flp and Cre, respectively, are two members of the tyrosine recombinase family derived from bacteriophages, that are used to mediate site-specific DNA insertions, deletions, and targeted expression of proteins in mammalian cell lines.
The recognition sites for Cre recombinase called LoxP...
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Related Experiment Video

Updated: Sep 9, 2025

Genome Editing in Mammalian Cell Lines using CRISPR-Cas
07:56

Genome Editing in Mammalian Cell Lines using CRISPR-Cas

Published on: April 11, 2019

22.0K

Advances in large-scale DNA engineering with the CRISPR system.

Lee Wha Gwon1,2, Isabel Wen Badon3,4, Youngjeon Lee1,2

  • 1National Primate Research Center, Korea Research Institute of Bioscience and Biotechnology, Cheongju, Republic of Korea.

Experimental & Molecular Medicine
|August 31, 2025
PubMed
Summary
This summary is machine-generated.

Clustered regularly interspaced short palindromic repeats (CRISPR)-based DNA insertion offers a streamlined, one-step method for in vivo gene editing. This advanced technology combines CRISPR-Cas with recombinases for accurate and efficient foreign DNA integration into target genes.

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Enhanced Genome Editing with Cas9 Ribonucleoprotein in Diverse Cells and Organisms
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A Universal Protocol for Large-scale gRNA Library Production from any DNA Source
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Related Experiment Videos

Last Updated: Sep 9, 2025

Genome Editing in Mammalian Cell Lines using CRISPR-Cas
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Genome Editing in Mammalian Cell Lines using CRISPR-Cas

Published on: April 11, 2019

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Enhanced Genome Editing with Cas9 Ribonucleoprotein in Diverse Cells and Organisms
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Enhanced Genome Editing with Cas9 Ribonucleoprotein in Diverse Cells and Organisms

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A Universal Protocol for Large-scale gRNA Library Production from any DNA Source
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A Universal Protocol for Large-scale gRNA Library Production from any DNA Source

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Area of Science:

  • Molecular Biology
  • Genetic Engineering
  • Biotechnology

Background:

  • Traditional DNA insertion methods often require pre-engineering recognition sequences or genetic crossing.
  • These conventional approaches can be inefficient and complex for in vivo gene modification.

Purpose of the Study:

  • To provide an overview of recent advancements in CRISPR-based target-specific DNA insertion technologies.
  • To discuss the potential future applications of these innovative gene editing tools.

Main Methods:

  • Integration of the CRISPR-Cas module with recombinase enzymes.
  • Development of one-step in vivo DNA insertion into target genes.

Main Results:

  • CRISPR-based gene insertion streamlines the engineering process.
  • Achieves accurate and efficient one-step foreign DNA insertion in vivo.

Conclusions:

  • CRISPR-based gene insertion represents a significant advancement over traditional methods.
  • This technology holds promise for diverse future applications in genetic engineering and beyond.