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Revealing Dentin Multiscale Structures Using High-Resolution Transmission Electron Microscopy.

M Leclercq1, M Vallet1,2, T Reiss1

  • 1Université Paris-Saclay, CentraleSupélec, ENS Paris-Saclay, CNRS, LMPS - Laboratoire de Mécanique Paris-Saclay, Gif-sur-Yvette, France.

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Summary
This summary is machine-generated.

This study reveals the nanoscale organization of collagen fibrils and hydroxyapatite crystals in dentin using high-resolution TEM. Findings clarify dentin

Keywords:
Fibrillar CollagensHydroxyapatiteNanostructureStructure-Properties RelationshipTooth componentsTropocollagen

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Area of Science:

  • Biomaterials Science
  • Nanotechnology
  • Dental Research

Background:

  • Dentin's microscale structure is well-understood via 2D microscopy.
  • 3D microstructural analysis revealed dentin's porous network.
  • Nanoscale organization of collagen fibrils (CFs) and mineral crystals in dentin remains unclear.

Purpose of the Study:

  • To analyze dentin nanostructure at the nanoscale, focusing on collagen fibril and mineral crystal organization.
  • To investigate dentin near the dentin-enamel junction (DEJ) and in the middle dentin.
  • To propose a protocol for TEM sample collection for investigating mineral/organic entanglement.

Main Methods:

  • High-resolution transmission electron microscopy (TEM).
  • Selected area electron diffraction.
  • TEM section orientation relative to the tubule axis.

Main Results:

  • Revealed woven entanglement of CFs and hydroxyapatite (HAP) minerals with varying isotropy.
  • Observed HAP crystals elongated along crystal axes, forming S-shaped structures around CFs.
  • Identified CFs parallel to the tubule axis in the transition zone, potentially explaining crack propagation.

Conclusions:

  • The study elucidates dentin's nanoscale structure, revealing mineral-organic entanglement.
  • Findings provide insights into structural gradients and the structure-properties relationship in dentin.
  • The proposed TEM protocol facilitates further investigation of dentin nanostructure.