Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

DNA Isolation01:34

DNA Isolation

DNA from cells is required for many biotechnology and research applications, such as molecular cloning. To remove and purify DNA from cells, researchers use various methods of DNA extraction. While the specifics of different protocols may vary, some general concepts underlie the process of DNA extraction.
DNA Isolation01:24

DNA Isolation

DNA isolation protocols can be fast and straightforward or complex and time-consuming depending on the type and quality of DNA required for further processing. For example, plasmid DNA extraction is a bit more complicated than genomic DNA extraction because of the need for an appropriate lysis method to separate plasmid DNA from gDNA during isolation. However, for specific applications, such as long-range DNA sequencing that require a good yield of high- quality DNA samples, we need to follow...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Geometrically Well-Controlled Wireframe RNA Nanostructures With Bundled-Helix Edges.

Small (Weinheim an der Bergstrasse, Germany)·2026
Same author

RNA nanostructures based on three-letter coding with non-canonical base pairs.

Nanoscale horizons·2026
Same author

Light-Up Nanostructures with Allosterically Controlled Fluorogenic DNA Aptamers.

Advanced materials (Deerfield Beach, Fla.)·2025
Same author

Ligation-induced DNA self-assembly.

Nucleic acids research·2025
Same author

DNA-programmed responsive microorganism assembly with controlled patterns and behaviors.

Science advances·2025
Same author

Automated design of scaffold-free DNA wireframe nanostructures.

Nature communications·2025
Same journal

Anion-Engineered Organic Electrochemical Transistors With Multi-Timescale Synaptic Dynamics for Task-Adaptive Spiking Neural Networks.

Small (Weinheim an der Bergstrasse, Germany)·2026
Same journal

Dimensional Effect on the Lattice Anharmonicity in Graphene and Graphite.

Small (Weinheim an der Bergstrasse, Germany)·2026
Same journal

A Modular Core-Shell Nanoparticle Platform for Dual-Modal MRI-Luminescence With High Relaxivity.

Small (Weinheim an der Bergstrasse, Germany)·2026
Same journal

Highly Selective Construction of D<sub>2</sub>-Symmetric Chiral Carbon Nanorings and the Diverse Assembly With Fullerenes.

Small (Weinheim an der Bergstrasse, Germany)·2026
Same journal

A Synergistic Process Optimization and Data-Driven Modeling Strategy for Unraveling and Enhancing the Low-Light Response in Back-Contact Solar Cells.

Small (Weinheim an der Bergstrasse, Germany)·2026
Same journal

Porous Hydrogel-Mediated One-Step Selection of Mannoprotein-Targeted Aptamers for Early Diagnosis of Invasive Saccharomyces cerevisiae Infections.

Small (Weinheim an der Bergstrasse, Germany)·2026
See all related articles

Related Experiment Video

Updated: May 15, 2026

Self-assembly of Complex Two-dimensional Shapes from Single-stranded DNA Tiles
10:23

Self-assembly of Complex Two-dimensional Shapes from Single-stranded DNA Tiles

Published on: May 8, 2015

11.8K

Glycerol-Assisted DNA Self-Assembly at Low Temperatures.

Zhengyang Sun1,2, Wen Wang3,4,5, Bryan Wei1,2

  • 1School of Life Sciences, Tsinghua University, Beijing, 100084, China.

Small (Weinheim an Der Bergstrasse, Germany)
|September 1, 2025
PubMed
Summary
This summary is machine-generated.

Researchers developed a new method for DNA nanostructure assembly using glycerol, enabling self-assembly at low temperatures. This technique is compatible with biomolecules, opening new avenues for molecular and cellular biology applications.

Keywords:
DNA nanostructuresglycerolself‐assembly

More Related Videos

Stable DNA Motifs, 1D and 2D Nanostructures Constructed from Small Circular DNA Molecules
09:32

Stable DNA Motifs, 1D and 2D Nanostructures Constructed from Small Circular DNA Molecules

Published on: April 12, 2019

6.5K
Self-Assembly of Gamma-Modified Peptide Nucleic Acids into Complex Nanostructures in Organic Solvent Mixtures
08:15

Self-Assembly of Gamma-Modified Peptide Nucleic Acids into Complex Nanostructures in Organic Solvent Mixtures

Published on: June 26, 2020

4.3K

Related Experiment Videos

Last Updated: May 15, 2026

Self-assembly of Complex Two-dimensional Shapes from Single-stranded DNA Tiles
10:23

Self-assembly of Complex Two-dimensional Shapes from Single-stranded DNA Tiles

Published on: May 8, 2015

11.8K
Stable DNA Motifs, 1D and 2D Nanostructures Constructed from Small Circular DNA Molecules
09:32

Stable DNA Motifs, 1D and 2D Nanostructures Constructed from Small Circular DNA Molecules

Published on: April 12, 2019

6.5K
Self-Assembly of Gamma-Modified Peptide Nucleic Acids into Complex Nanostructures in Organic Solvent Mixtures
08:15

Self-Assembly of Gamma-Modified Peptide Nucleic Acids into Complex Nanostructures in Organic Solvent Mixtures

Published on: June 26, 2020

4.3K

Area of Science:

  • Biotechnology
  • Nanotechnology
  • Molecular Biology

Background:

  • Thermal annealing is standard for DNA nanostructure assembly but requires high temperatures unsuitable for biomolecular applications.
  • Physiological or lower temperatures are preferred for integrating DNA nanostructures with biological systems.

Purpose of the Study:

  • To develop a novel method for DNA self-assembly at arbitrary temperatures, including freezing conditions.
  • To assess the compatibility of this new method with natural biomolecules.

Main Methods:

  • Supplementing glycerol into the DNA self-assembly reaction system.
  • Conducting DNA nanostructure production at various temperatures, including sub-zero levels.
  • Performing co-assembly tests with proteins from cell lysate.

Main Results:

  • Successful generation of diverse DNA nanostructures using glycerol at arbitrary temperatures.
  • Achieved unprecedented low-temperature DNA nanostructure assembly, down to freezing levels.
  • Demonstrated perfect compatibility of glycerol-based DNA nanostructures with functioning guest proteins.

Conclusions:

  • Glycerol enables efficient DNA nanostructure self-assembly at low, arbitrary temperatures.
  • The method is compatible with natural biomolecules, facilitating applications in molecular and cellular biology.
  • This approach offers new opportunities for designing and utilizing DNA nanostructures in biological contexts.