Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Incomplete Dominance01:43

Incomplete Dominance

25.4K
Gregor Mendel's work (1822 - 1884) was primarily focused on pea plants. Through his initial experiments, he determined that every gene in a diploid cell has two variants called alleles inherited from each parent. He suggested that amongst these two alleles, one allele is dominant in character and the other recessive. The combination of alleles determines the phenotype of a gene in an organism.
25.4K
Next-generation Sequencing03:00

Next-generation Sequencing

92.6K
The first human genome sequencing project cost $2.7 billion and was declared complete in 2003, after 15 years of international cooperation and collaboration between several research teams and funding agencies. Today, with the advent of next-generation sequencing technologies, the cost and time of sequencing a human genome have dropped over 100 fold.
Next-Generation Sequencing Methods
Although all next-generation methods use different technologies, they all share a set of standard features....
92.6K
Maxam-Gilbert Sequencing01:05

Maxam-Gilbert Sequencing

11.4K
In the same year as the discovery of the Sanger sequencing method, another group of scientists, Allan Maxam and Walter Gilbert, demonstrated their chemical-cleavage method for DNA sequencing. The Maxam-Gilbert method relies on using different chemicals that can cleave the DNA sequence at specific sites, the separation of resulting DNA fragments of variable size using electrophoresis, and deciphering the DNA sequence from the resulting gel bands.
Challenges of the Maxam-Gilbert Method
The...
11.4K
Evolutionary Relationships through Genome Comparisons02:54

Evolutionary Relationships through Genome Comparisons

6.1K
Genome comparison is one of the excellent ways to interpret the evolutionary relationships between organisms. The basic principle of genome comparison is that if two species share a common feature, it is likely encoded by the DNA sequence conserved between both species. The advent of genome sequencing technologies in the late 20th century enabled scientists to understand the concept of conservation of domains between species and helped them to deduce evolutionary relationships across diverse...
6.1K
Pedigree Analysis01:35

Pedigree Analysis

85.1K
Overview
85.1K
RNA-seq03:21

RNA-seq

10.4K
RNA sequencing, or RNA-Seq, is a high-throughput sequencing technology used to study the transcriptome of a cell. Transcriptomics helps to interpret the functional elements of a genome and identify the molecular constituents of an organism. Additionally, it also helps in understanding the development of an organism and the occurrence of diseases. 
Before the discovery of RNA-seq, microarray-based methods and Sanger sequencing were used for transcriptome analysis. However, while...
10.4K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Quantifying phenotypic and genetic variation for cow fertility phenotypes in American Simmental using total herd reporting data.

Journal of animal science·2024
Same author

Uncovering the architecture of selection in two <i>Bos taurus</i> cattle breeds.

Evolutionary applications·2024
Same author

Exploring genetic variation and population structure in a threatened species, Noturus placidus, with whole-genome sequence data.

G3 (Bethesda, Md.)·2022
Same author

Genetic ancestry, admixture, and population structure in rural Dominica.

PloS one·2021
Same author

Powerful detection of polygenic selection and evidence of environmental adaptation in US beef cattle.

PLoS genetics·2021
Same author

Genome-Wide Association Analyses of Fertility Traits in Beef Heifers.

Genes·2021

Related Experiment Video

Updated: Sep 9, 2025

Candidate Gene Testing in Clinical Cohort Studies with Multiplexed Genotyping and Mass Spectrometry
05:53

Candidate Gene Testing in Clinical Cohort Studies with Multiplexed Genotyping and Mass Spectrometry

Published on: June 21, 2018

10.2K

A primer on sequencing and genotype imputation in cattle.

Troy N Rowan1,2

  • 1Department of Animal Science, University of Tennessee, Knoxville, TN, USA37996.

Journal of Animal Science
|September 1, 2025
PubMed
Summary

Genotyping technologies like SNP arrays accelerate beef industry improvements. Low-cost sequencing and imputation offer more genomic data for discovery and routine genotyping.

Keywords:
GWAScattlegenomic predictionimputationsequencing

More Related Videos

Infinium Assay for Large-scale SNP Genotyping Applications
13:33

Infinium Assay for Large-scale SNP Genotyping Applications

Published on: November 19, 2013

39.2K
Transcriptome Profiling of In-Vivo Produced Bovine Pre-implantation Embryos Using Two-color Microarray Platform
09:04

Transcriptome Profiling of In-Vivo Produced Bovine Pre-implantation Embryos Using Two-color Microarray Platform

Published on: January 30, 2017

7.9K

Related Experiment Videos

Last Updated: Sep 9, 2025

Candidate Gene Testing in Clinical Cohort Studies with Multiplexed Genotyping and Mass Spectrometry
05:53

Candidate Gene Testing in Clinical Cohort Studies with Multiplexed Genotyping and Mass Spectrometry

Published on: June 21, 2018

10.2K
Infinium Assay for Large-scale SNP Genotyping Applications
13:33

Infinium Assay for Large-scale SNP Genotyping Applications

Published on: November 19, 2013

39.2K
Transcriptome Profiling of In-Vivo Produced Bovine Pre-implantation Embryos Using Two-color Microarray Platform
09:04

Transcriptome Profiling of In-Vivo Produced Bovine Pre-implantation Embryos Using Two-color Microarray Platform

Published on: January 30, 2017

7.9K

Area of Science:

  • Animal Genomics
  • Agricultural Biotechnology
  • Population Genetics

Background:

  • Genotyping technologies have revolutionized the beef industry, enabling genomic selection.
  • Single nucleotide polymorphism (SNP) arrays have been central to genomic selection programs.
  • Advancements in sequencing and imputation present cost-effective alternatives to arrays.

Purpose of the Study:

  • To review array-based genotyping, genomic sequencing, and low-pass sequencing with imputation.
  • To explore the potential of low-coverage sequencing and imputation for genomic discovery and routine genotyping.

Main Methods:

  • Overview of current genotyping technologies.
  • Discussion of sequencing and imputation methodologies.
  • Analysis of cost-effectiveness and information yield.

Main Results:

  • Sequencing and imputation offer significantly more data than arrays at a lower cost.
  • Low-coverage sequencing combined with imputation is a promising approach.
  • Declining sequencing costs enhance the feasibility of these advanced methods.

Conclusions:

  • Low-coverage sequencing and imputation can drive genomic discovery in beef populations.
  • These methods are poised to become standard for routine genotyping.
  • Technological advancements promise accelerated genetic improvement in livestock.