Related Experiment Video
Updated: Aug 12, 2026

Culturing Primary Rat Inner Medullary Collecting Duct Cells
Published on: June 21, 2013
Osmoregulation of renal papillary cells
Abstract:
Element concentrations were determined in various extra- and intracellular compartments of the rat renal papilla in antidiuresis and after furosemide-induced diuresis using electron microprobe analysis to elucidate further how the cells adapt osmotically to different osmolalities. In antidiuresis and diuresis the sum of intracellular cations (sodium and potassium), accompanying anions and urea was insufficient in both cases to provide cell osmolalities similar to those in extracellular compartments. This finding provides further evidence that the papillary cells achieve osmoadaptation to widely differing extracellular electrolyte concentrations mainly by varying the cellular concentrations of osmotically-active substances other than urea and electrolytes.
Related Concept Videos
Hormonal Regulation
Introduction to Urinary System
The kidneys are bean-shaped organs located in the retroperitoneal space, on either side of the vertebral column, between the T12 and L3 vertebrae. They are partially protected by the rib cage and surrounded by perirenal fat, which provides cushioning. They are responsible for urine formation and play critical roles in regulating blood pressure, electrolyte levels, and hormone production. The ureters...
Reabsorption and Secretion in the DCT and Collecting Duct
The distal part of the DCT, along with the...
Renal Regulation of Acid-Base Balance
In the kidneys, cells within the proximal convoluted tubules (PCT) and the collecting ducts secrete hydrogen ions (H+) into the tubular fluid. Specifically, in the PCT, Na+/H+ antiporters secrete H+ while reabsorbing Na+.
However, the intercalated cells in...
Physiology of the Genitourinary System II: Tubular Reabsorption and Secretion
Physiology of the Genitourinary System III: Urine Concentration and Dilution

