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Functional analysis of tight junction organization.

D R DiBona

    Pflugers Archiv : European Journal of Physiology
    |January 1, 1985
    PubMed
    Summary
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    Tight junctions act as multicompartment barriers controlling paracellular transport. Their structure and function are best understood as polarized, with lipid components playing a key role in organization.

    Area of Science:

    • Cell biology
    • Biophysics

    Background:

    • Tight junctions form a rate-limiting barrier to paracellular transport.
    • Understanding their structure-function relationship requires considering them as multicompartment systems.

    Purpose of the Study:

    • To examine the functional basis of tight junction design.
    • To correlate tight junction structure and function under varying conditions.

    Main Methods:

    • Analysis of osmotic sensitivity.
    • Voltage-clamping protocols to study transmural potential difference.
    • Examination of experimentally-induced junction deformation.

    Main Results:

    • Osmotic and electrical gradient reversals induce parallel changes in tight junction structure and function.

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  • Tight junctions exhibit functionally polarized and rectifying properties.
  • Freeze-fracture analysis suggests lipidic rather than proteinaceous components are primary in intramembranous organization.

    • Conclusions:

    • Tight junctions function as multicompartment systems sensitive to osmotic and electrical gradients.
    • Functional polarization and rectification are key properties of tight junctions.
    • Lipidic components are likely central to the intramembranous organization of tight junctions.