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Nested PCR detection of JC polyomavirus large T-antigen in prostate cancer tissues: a case-control analysis in a Sudanese population

  • 0Institute of Medical Research, Al-Neelain University, Sudan, Khartoum, Sudan.
Journal of the Egyptian National Cancer Institute +

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September 1, 2025

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September 1, 2025

View abstract on PubMed

Summary

This summary is machine-generated.

JC polyomavirus (JCPyV) large T-antigen DNA was found more frequently in prostate cancer (PCa) tissues than in benign prostatic hyperplasia tissues. This suggests a potential link between JCPyV infection and increased prostate cancer risk.

Area Of Science

  • Oncology
  • Virology
  • Molecular Biology

Background

  • The role of JC polyomavirus (JCPyV) in prostate cancer (PCa) etiology is debated due to conflicting in vitro findings.
  • Understanding viral oncogenic mechanisms is crucial for elucidating PCa development.

Purpose Of The Study

  • To investigate the association between JCPyV infection and prostate cancer.
  • To detect the JCPyV large T-antigen gene in prostate tissue specimens.

Main Methods

  • A case-control study involving 100 participants (50 PCa, 50 benign prostatic hyperplasia).
  • Nested polymerase chain reaction (PCR) was used to detect JCPyV large T-antigen DNA in formalin-fixed paraffin-embedded (FFPE) prostate tissues.
  • Logistic regression analysis was employed to assess the association between JCPyV presence and PCa.

Main Results

  • JCPyV large T-antigen DNA was detected in 58% of PCa cases versus 38% of controls (P=0.045).
  • The odds ratio for PCa associated with JCPyV presence was 1.45 (95% CI: 1.011 to 5.019).
  • JCPyV T-antigen positive PCa patients were significantly older than T-antigen negative patients (73.3 vs 67.0 years, P=0.029).

Conclusions

  • A significantly higher prevalence of JCPyV large T-antigen gene was observed in prostate cancer patients.
  • These findings suggest a potential link between JCPyV infection and an increased risk of prostate cancer.
  • Further research is warranted to explore the molecular mechanisms and clinical implications of this association.

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