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An electrochemical sensor based on a triblock polyadenine-based probe and copper nanoclusters for the robust detection of the HPV16 gene

  • 0School of Materials Science and Engineering, Xiangtan University, Xiangtan 411105, PR China. yupeng@xtu.edu.cn.
Analytical Methods : Advancing Methods and Applications +

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September 1, 2025

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September 1, 2025

View abstract on PubMed

Summary

This summary is machine-generated.

A new electrochemical sensor detects human papillomavirus type 16 (HPV16), a biomarker for cervical cancer. This sensitive HPV16 detection method offers advancements in early diagnosis and prevention strategies.

Area Of Science

  • Biomedical Engineering
  • Nanotechnology
  • Molecular Diagnostics

Background

  • Cervical cancer is a significant global health concern for women.
  • Human papillomavirus type 16 (HPV16) is a primary etiological factor in cervical cancer development.
  • Sensitive and selective detection of HPV16 is crucial for early diagnosis and effective prevention.

Purpose Of The Study

  • To develop a novel electrochemical sensor for the rapid, sensitive, and selective detection of HPV16.
  • To utilize a triblock polyadenine-based probe (TPP) and copper nanoclusters (CuNCs) for HPV16 detection.
  • To provide a valuable tool for cervical cancer prevention and electrochemical biosensor development.

Main Methods

  • Construction of an electrochemical sensor using a TPP with a central polyadenine segment and flanking DNA probes.
  • Immobilization of the TPP onto a gold electrode surface.
  • Hybridization of a DNA template with TPP probes to mediate CuNC formation and signal generation.
  • Detection of HPV16 by its binding to TPP probes, leading to DNA template release and signal change.

Main Results

  • The sensor achieved a limit of detection (LOD) of 3.34 pM for HPV16.
  • A linear detection range for HPV16 was established from 10 pM to 10 μM.
  • The sensor exhibited good sensitivity, selectivity, and satisfactory recovery rates.

Conclusions

  • The developed electrochemical sensor enables sensitive and selective detection of HPV16.
  • This biosensor design offers a promising approach for early cervical cancer diagnosis.
  • The findings contribute to the advancement of electrochemical biosensor technology for disease detection.