Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Imaging Studies III: Gastrointestinal Motility Studies and Virtual Colonoscopy01:26

Imaging Studies III: Gastrointestinal Motility Studies and Virtual Colonoscopy

142
This lesson explores three gastrointestinal imaging techniques: radionuclide testing, colonic transit studies, and virtual colonoscopy.
Radionuclide Testing
Radionuclide testing is a sophisticated medical technique for assessing gastrointestinal motility. It focuses on gastric emptying and colonic transit time. Radioactive markers track the movement of food through the digestive system, providing insights into gastrointestinal disorders.
In gastric emptying studies, a meal's liquid and...
142

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Epithelial regeneration in the gastrointestinal tract.

Current opinion in genetics & development·2026
Same author

Compression benchmarking of holotomography data using OME-Zarr format.

PloS one·2026
Same author

GIGANTEA shapes diurnal seedling growth by sequestering SMAX1 and SMXL2.

The Plant cell·2026
Same author

Growth-Pathway-Controlled van der Waals Epitaxy of Phase-Selective Tin Sulfides.

Advanced science (Weinheim, Baden-Wurttemberg, Germany)·2026
Same author

Refractive Index-Correlated Pseudocoloring for Adaptive Color Fusion in Holotomographic Cytology.

Cytometry. Part A : the journal of the International Society for Analytical Cytology·2026
Same author

Recurrent COPA mutation drives R-spondin-independent Wnt activation in intestinal tumors.

Nature genetics·2026

Related Experiment Video

Updated: Sep 9, 2025

Label-free, High-Resolution 3D Imaging and Machine Learning Analysis of Intestinal Organoids via Low-Coherence Holotomography
10:40

Label-free, High-Resolution 3D Imaging and Machine Learning Analysis of Intestinal Organoids via Low-Coherence Holotomography

Published on: August 12, 2025

371

Label-free, High-Resolution 3D Imaging and Machine Learning Analysis of Intestinal Organoids via Low-Coherence

Jimin Cho1, Mahn Jae Lee2, Juyeon Park3

  • 1Graduate School of Stem Cell and Regenerative Biology, Korea Advanced Institute of Science and Technology (KAIST).

Journal of Visualized Experiments : Jove
|September 1, 2025
PubMed
Summary

This study introduces holotomography (HT) for label-free, 3D imaging of intestinal organoids. This method quantifies organoid properties and enables high-throughput analysis for regenerative medicine and drug screening.

More Related Videos

Single-Cell Resolution Three-Dimensional Imaging of Intact Organoids
10:40

Single-Cell Resolution Three-Dimensional Imaging of Intact Organoids

Published on: June 5, 2020

16.6K
Three-Dimensional Imaging of Organoids to Study Primary Ciliogenesis During ex vivo Organogenesis
06:57

Three-Dimensional Imaging of Organoids to Study Primary Ciliogenesis During ex vivo Organogenesis

Published on: May 14, 2021

1.7K

Related Experiment Videos

Last Updated: Sep 9, 2025

Label-free, High-Resolution 3D Imaging and Machine Learning Analysis of Intestinal Organoids via Low-Coherence Holotomography
10:40

Label-free, High-Resolution 3D Imaging and Machine Learning Analysis of Intestinal Organoids via Low-Coherence Holotomography

Published on: August 12, 2025

371
Single-Cell Resolution Three-Dimensional Imaging of Intact Organoids
10:40

Single-Cell Resolution Three-Dimensional Imaging of Intact Organoids

Published on: June 5, 2020

16.6K
Three-Dimensional Imaging of Organoids to Study Primary Ciliogenesis During ex vivo Organogenesis
06:57

Three-Dimensional Imaging of Organoids to Study Primary Ciliogenesis During ex vivo Organogenesis

Published on: May 14, 2021

1.7K

Area of Science:

  • Biophysics
  • Cell Biology
  • Regenerative Medicine

Background:

  • Accurate imaging of intestinal organoids is vital for research.
  • Current methods may involve fluorescent markers, causing phototoxicity.
  • Label-free imaging preserves organoid integrity and function.

Purpose of the Study:

  • To detail a protocol for label-free, 3D imaging of organoids using holotomography (HT).
  • To enable high-throughput, quantitative analysis of organoid properties.
  • To support applications in disease modeling and drug screening.

Main Methods:

  • Utilizing low-coherence holotomography (HT) for high-resolution, 3D visualization.
  • Employing real-time phase-based imaging for label-free tracking.
  • Applying machine learning for segmentation and feature extraction of imaging data.

Main Results:

  • HT provides label-free, quantitative assessment of organoid biophysical properties (volume, protein density).
  • Machine learning integration allows for consistent, high-throughput analysis.
  • The protocol enables unbiased evaluation of organoid viability, structure, and drug response.

Conclusions:

  • This protocol offers a reproducible method for organoid imaging and analysis.
  • Holotomography facilitates real-time monitoring of morphological changes at subcellular resolution.
  • The approach is broadly applicable to various organoid systems in research and development.